Journal article
Cloning and expression of amino acid transporters from broad bean
Plant molecular biology, Vol.41(2), pp.259-268
09/1999
Handle:
https://hdl.handle.net/2376/108061
PMID: 10579492
Abstract
This work describes the isolation of a full-length (VfAAP2) and three partial amino acid transporter genes (VfAAPa, VfAAPb, VfAAPc) from broad bean (Vicia faba L.). The function of VfAAP2 was tested by heterologous expression in a yeast mutant deficient in proline uptake. VfAAP2 mediates proton-dependent proline uptake with an apparent Km of about 1 mM. Analysis of substrate specificity by competition experiments showed that aromatic amino acids, neutral aliphatic acids and L-citrulline are the best competitors, whereas basic amino acids do not compete with proline. Northern analysis indicates that all VfAAPs exhibit different patterns of expression. VfAAP2 is most strongly expressed in the stem and at a lower level in sink leaves and pods. VfAAPa, VfAAPb and VfAAPc are most strongly expressed in the flowers, but their expression in the other organs varies.
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Details
- Title
- Cloning and expression of amino acid transporters from broad bean
- Creators
- Florence Montamat - Laboratoire de Physiologie et Biochimie Végétales, ERS CNRS 6099, B^atiment Botanique, UFR Sciences Université de Poitiers 40 avenue du Recteur Pineau 86022 Poitiers Cédex FranceLaurence Maurousset - Laboratoire de Physiologie et Biochimie Végétales, ERS CNRS 6099, B^atiment Botanique, UFR Sciences Université de Poitiers 40 avenue du Recteur Pineau 86022 Poitiers Cédex FranceMechthild Tegeder - Plant Physiology, Zentrum für Molekularbiologie der Pflanzen (ZMBP) Universität Tübingen, Auf der Morgenstelle 1 72076 Tübingen GermanyWolf Frommer - Plant Physiology, Zentrum für Molekularbiologie der Pflanzen (ZMBP) Universität Tübingen, Auf der Morgenstelle 1 72076 Tübingen GermanySerge Delrot - Laboratoire de Physiologie et Biochimie Végétales, ERS CNRS 6099, B^atiment Botanique, UFR Sciences Université de Poitiers 40 avenue du Recteur Pineau 86022 Poitiers Cédex France
- Publication Details
- Plant molecular biology, Vol.41(2), pp.259-268
- Academic Unit
- Biological Sciences, School of
- Publisher
- Kluwer Academic Publishers; Dordrecht
- Identifiers
- 99900547486701842
- Language
- English
- Resource Type
- Journal article