Journal article
Cloning, characterization and genomic structure of the natural killer cell enhancement factor ( NKEF)-like gene from homozygous clones of rainbow trout ( Oncorhynchus mykiss)
Developmental and comparative immunology, Vol.25(1), pp.25-35
2001
Handle:
https://hdl.handle.net/2376/105924
PMID: 10980317
Abstract
Natural killer cell enhancement factor (NKEF) belongs to the antioxidant protein family. In the human, NKEF has the ability to enhance natural killer cell cytotoxic activity in vitro. In the present work, the cDNAs of NKEF from three strains of homozygous clones of rainbow trout were cloned from the splenic cDNA library of one of the strains, OSU142, and then by RT-PCR for the Hot Creek (HC) and Arlee (AR) strains. The HC sequence has 99% sequence identity with both OSU142 and AR. OSU142 and AR have only one nucleotide difference in the cDNA sequence. All three sequences have the same deduced NKEF peptide, which contains 199 amino acids. The 6.5 kb genomic DNA of OSU142 containing NKEF was sequenced and contains six exons and five introns. Tissue specific expression of
NKEF was studied by RT-PCR in eight different tissues of OSU142 and revealed that all tissues expressed
NKEF. A southern blot revealed that the gene for NKEF is present in a single copy. The cDNA and amino acid sequences of trout NKEF have high similarity with human, rat, mouse and carp sequences, therefore, indicating that
NKEF is a very conserved gene.
Metrics
5 Record Views
Details
- Title
- Cloning, characterization and genomic structure of the natural killer cell enhancement factor ( NKEF)-like gene from homozygous clones of rainbow trout ( Oncorhynchus mykiss)
- Creators
- Hui Zhang - Department of Animal Sciences, Washington State University, 114 New Experimental Animal Laboratory Building, Pullman WA 99164-6351, USAJason P Evenhuis - Department of Animal Sciences, Washington State University, 114 New Experimental Animal Laboratory Building, Pullman WA 99164-6351, USAGary H Thorgaard - School of Biological Sciences, Washington State University, Pullman WA 99164-4236, USASandra S Ristow - Department of Animal Sciences, Washington State University, 114 New Experimental Animal Laboratory Building, Pullman WA 99164-6351, USA
- Publication Details
- Developmental and comparative immunology, Vol.25(1), pp.25-35
- Academic Unit
- Pharmaceutical Sciences, Department of; Biological Sciences, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546990201842
- Language
- English
- Resource Type
- Journal article