Journal article
Cloning of bovine CD69
Veterinary immunology and immunopathology, Vol.88(1), pp.43-48
2002
Handle:
https://hdl.handle.net/2376/116218
PMID: 12088643
Abstract
CD69 is rapidly inducible on various hematopoietic cells upon stimulation and is detectable as an early activation antigen. Although CD69 is well characterized in human and mouse, no information is available on bovine CD69. We report here that, bovine CD69 was cloned from a cDNA expression library prepared from activated peripheral blood lymphocytes. The full-length cDNA contained an 80
bp 5′ untranslated region, followed by a 600
bp coding region and AU-rich motifs in a 3′ untranslated region (GenBank accession number AF272828). Comparison of the bovine CD69 coding sequence reveals 69.4 and 78.2% nucleotide sequence identities with mouse and human CD69, respectively. The predicted amino acid sequence of bovine CD69 shares 56.3 and 62.3% sequence identity when compared with mouse and human CD69, respectively. Bovine CD69 has the highly conserved amino acid sequences found in the C-type lectin family, suggesting that the conserved residues may be important for conformation and binding to the, as yet unidentified ligand. In addition, the cytoplasmic tail of bovine CD69 has two casein kinase-2 (CK-2) phosphorylation sites. These data suggest that bovine CD69 plays an important role in the activation of lymphocytes.
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Details
- Title
- Cloning of bovine CD69
- Creators
- J.S Ahn - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAM.J Hamilton - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAW.C Davis - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAY.H Park - Department of Microbiology, School of Agricultural Biotechnology, College of Veterinary Medicine, Seoul National University, Seo-Doon Dong 103, Kwon-Sun Gu, 441-744 Suwon, South Korea
- Publication Details
- Veterinary immunology and immunopathology, Vol.88(1), pp.43-48
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548103001842
- Language
- English
- Resource Type
- Journal article