Journal article
Cloning of the cDNA for U1 small nuclear ribonucleoprotein particle 70K protein from Arabidopsis thaliana
Biochimica et biophysica acta. Gene structure and expression, Vol.1171(1), pp.88-92
1992
Handle:
https://hdl.handle.net/2376/115472
PMID: 1420366
Abstract
We cloned and sequenced a plant cDNA that encodes U1 small nuclear ribonucleoprotein (snRNP) 70K protein. The plant U1 snRNP 70K protein cDNA is not full length and lacks the coding region for 68 amino acids in the amino-terminal region as compared to human U1 snRNP 70K protein. Comparison of the deduced amino acid sequence of the plant U1 snRNP 70K protein with the amino acid sequence of animal and yeast U1 snRNP 70K protein showed a high degree of homology. The plant U1 snRNP 70K protein is more closely related to the human counter part than to the yeast 70K protein. The carboxy-terminal half is less well conserved but, like the vertebrate 70K proteins, is rich in charged amino acids. Northern analysis with the RNA isolated from different parts of the plant indicates that the snRNP 70K gene is expressed in all of the parts tested. Southern blotting of genomic DNA using the cDNA indicates that the U1 snRNP 70K protein is coded by a single gene.
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Details
- Title
- Cloning of the cDNA for U1 small nuclear ribonucleoprotein particle 70K protein from Arabidopsis thaliana
- Creators
- A.S.N Reddy - Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA USAAndrew J Czernik - Department of Molecular and Cellular Neurosciences, Rockefeller University, New York, NY USAGynheung An - Institute of Biological Chemistry, Washington State University, Pullman, WA USAB.W Poovaiah - Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA USA
- Publication Details
- Biochimica et biophysica acta. Gene structure and expression, Vol.1171(1), pp.88-92
- Academic Unit
- Horticulture, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548591401842
- Language
- English
- Resource Type
- Journal article