Journal article
Cloning of the mating type locus from Ascochyta lentis (teleomorph: Didymella lentis) and development of a multiplex PCR mating assay for Ascochyta species
Current genetics, Vol.50(3), pp.203-215
09/2006
Handle:
https://hdl.handle.net/2376/109927
PMID: 16847660
Abstract
The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers were designed to specifically amplify MAT from several Ascochyta spp. including A. pisi, A. fabae and A. viciae-villosae in addition to A. lentis. Four hundred and fifty and 700 bp fragments were amplified from MAT1-1 and MAT1-2 isolates, respectively, and fragment size correlated perfectly with laboratory crosses using mating type tester strains. MAT-specific PCR allowed rapid scoring of mating type in crude DNA extracts from geographically diverse population samples of A. viciae-villosae from California and Washington State, USA. This co-dominant MAT-specific PCR assay will be a valuable tool for studying the population structure, biology and epidemiology of these fungi.
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Details
- Title
- Cloning of the mating type locus from Ascochyta lentis (teleomorph: Didymella lentis) and development of a multiplex PCR mating assay for Ascochyta species
- Creators
- Mohamed Chérif - Laboratoire de Phytopathologie Institut National Agronomique de Tunisie 43 Avenue Charles Nicolle 1082, Cité Mahrajéne Tunis TunisiaMartin Chilvers - Department of Plant Pathology Washington State University Pullman WA 99164-6430 USAHajime Akamatsu - Department of Plant Pathology Washington State University Pullman WA 99164-6430 USATobin Peever - Department of Plant Pathology Washington State University Pullman WA 99164-6430 USAWalter Kaiser - 3394 Chickory Way Boise ID 83706 USA
- Publication Details
- Current genetics, Vol.50(3), pp.203-215
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900547369901842
- Language
- English
- Resource Type
- Journal article