Cloning, sequencing, and expression of a gene from Campylobacter jejuni encoding a protein (Omp18) with similarity to peptidoglycan-associated lipoproteins

M E Konkel; D J Mead; W Cieplak, Jr

doi:10.1128/IAI.64.5.1850-1853.1996

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Cloning, sequencing, and expression of a gene from Campylobacter jejuni encoding a protein (Omp18) with similarity to peptidoglycan-associated lipoproteins

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Cloning, sequencing, and expression of a gene from Campylobacter jejuni encoding a protein (Omp18) with similarity to peptidoglycan-associated lipoproteins

M E Konkel, D J Mead and W Cieplak, Jr

Infection and immunity, Vol.64(5), pp.1850-1853

05/1996

DOI: https://doi.org/10.1128/IAI.64.5.1850-1853.1996

Handle:

https://hdl.handle.net/2376/109305

PMCID: PMC174003

PMID: 8613402

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https://doi.org/10.1128/IAI.64.5.1850-1853.1996View

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Abstract

Amino Acid Sequence

Genes, Bacterial

Bacterial Outer Membrane Proteins - immunology

Lipoproteins - genetics

Humans

Bacterial Proteins - genetics

Molecular Sequence Data

Chromosome Mapping

Peptidoglycan - genetics

Peptidoglycan - immunology

Campylobacter Infections - prevention & control

Sequence Homology, Amino Acid

Campylobacter Infections - immunology

DNA, Bacterial - genetics

Campylobacter jejuni - immunology

Base Sequence

Cloning, Molecular

Bacterial Proteins - immunology

Gram-Negative Bacteria - genetics

Lipoproteins - immunology

Bacterial Outer Membrane Proteins - genetics

Campylobacter jejuni - genetics

A Campylobacter jejuni genomic plasmid library was screened with antiserum generated against whole C. jejuni, revealing two immunoreactive clones. Sequence analysis of the recombinant plasmids revealed a common open reading frame of 498 nucleotides encoding a protein of 165 amino acids with a calculated molecular mass of 18,018 Da. The recombinant product partitioned to the outer membrane fractions of Escherichia coli transformants and has been designated Omp18. The deduced amino acid sequence of the cloned C. jejuni gene exhibits considerable similarity to peptidoglycan-associated lipoproteins from other gram-negative bacteria.

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Title: Cloning, sequencing, and expression of a gene from Campylobacter jejuni encoding a protein (Omp18) with similarity to peptidoglycan-associated lipoproteins
Creators: M E Konkel - Department of Microbiology, Washington State University, Pullman 99164, USA. Konkel@mail.wsu.edu
D J Mead
W Cieplak, Jr
Publication Details: Infection and immunity, Vol.64(5), pp.1850-1853
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Identifiers: 99900547564001842
Language: English
Resource Type: Journal article