Journal article
Co-cultivation of Trichoderma reesei RutC30 with three black Aspergillus strains facilitates efficient hydrolysis of pretreated wheat straw and shows promises for on-site enzyme production
Bioresource technology, Vol.169, pp.143-148
10/2014
Handle:
https://hdl.handle.net/2376/112839
PMID: 25043347
Abstract
•Co-cultivation of Trichoderma reesei with novel Aspergillus saccharolyticus.•80% efficiency of hydrolysis using crude enzymes from mixed cultures.•Solid-state fermentation being well-suited for co-cultivation of selected strains.
Co-cultivation of fungi may be an excellent system for on-site production of cellulolytic enzymes in a single bioreactor. Enzyme supernatants from mixed cultures of Trichoderma reesei RutC30, with either the novel Aspergillus saccharolyticus AP, Aspergillus carbonarius ITEM 5010 or Aspergillus niger CBS 554.65 cultivated in solid-state fermentation were tested for avicelase, FPase, endoglucanase and beta-glucosidase activity as well as in hydrolysis of pretreated wheat straw. Around 30% more avicelase activity was produced in co-cultivation of T. reesei and A. saccharolyticus than in T. reesei monoculture, suggesting synergistic interaction between those fungi. Fermentation broths of mixed cultures of T. reesei with different Aspergillus strains resulted in approx. 80% efficiency of hydrolysis which was comparable to results obtained using blended supernatants from parallel monocultures. This indicates that co-cultivation of T. reesei with A. saccharolyticus or A. carbonarius could be a competitive alternative for monoculture enzyme production and a cheaper alternative to commercial enzymes.
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Details
- Title
- Co-cultivation of Trichoderma reesei RutC30 with three black Aspergillus strains facilitates efficient hydrolysis of pretreated wheat straw and shows promises for on-site enzyme production
- Creators
- Marta Kolasa - Section for Sustainable Biotechnology, Aalborg University Copenhagen, A. C. Meyers Vænge 15, DK-2450 Copenhagen SV, DenmarkBirgitte Kiær Ahring - Section for Sustainable Biotechnology, Aalborg University Copenhagen, A. C. Meyers Vænge 15, DK-2450 Copenhagen SV, DenmarkPeter Stephensen Lübeck - Section for Sustainable Biotechnology, Aalborg University Copenhagen, A. C. Meyers Vænge 15, DK-2450 Copenhagen SV, DenmarkMette Lübeck - Section for Sustainable Biotechnology, Aalborg University Copenhagen, A. C. Meyers Vænge 15, DK-2450 Copenhagen SV, Denmark
- Publication Details
- Bioresource technology, Vol.169, pp.143-148
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547326001842
- Language
- English
- Resource Type
- Journal article