Journal article
Colloidal gold nanoparticle probe-based immunochromatographic assay for the rapid detection of chromium ions in water and serum samples
Analytica chimica acta, Vol.745, pp.99-105
10/01/2012
Handle:
https://hdl.handle.net/2376/103511
PMCID: PMC3468954
PMID: 22938612
Abstract
[Display omitted]
► An immunochromatography assay (ICA) is the first time applied for Cr detection. ► The ICA combine with a colorimetric reader is developed for quantification of Cr. ► The pretreatment helps to measure chromium speciation in water and serum samples.
An immunochromatographic assay (ICA) using gold nanoparticles coated with monoclonal antibody (McAb) for the detection of chromium ions (Cr) in water and serum samples was developed, optimized and validated. Gold nanoparticles coated with affinity-purified monoclonal antibodies against isothiocyanobenzyl-EDTA (iEDTA)-chelated Cr3+ were used as the detecting reagent in this completive immunoassay-based one-step test strip. The ICA was investigated to measure chromium speciation (Cr3+ and Cr6+ ions) in water samples. Chromium standard samples of 0–80ngmL−1 in water were determined by the test strips. The results showed that the visual lowest detection limit (LDL) of the test strip was 50.0ngmL−1. A portable colorimetric lateral flow reader was used for the quantification of Cr. The results indicated that the linear range of the ICA with colorimetric detection was 5–80ngmL−1. The ICA was also validated for the detection of chromium ions in serum samples. The test trips showed high stability in that they could be stored at 37°C for at least 12 weeks without significant loss of activity. The test strip also showed good selectivity for Cr detection with negligible interference from other heavy metals. Because of its low cost and short testing time (within 5min), the test strip is especially suitable for on-site large-scale screening of Cr-polluted water samples, biomonitoring of Cr exposure, and many other field applications.
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Details
- Title
- Colloidal gold nanoparticle probe-based immunochromatographic assay for the rapid detection of chromium ions in water and serum samples
- Creators
- Xi Liu - Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou 510632, PR ChinaJun-Jian Xiang - Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou 510632, PR ChinaYong Tang - Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou 510632, PR ChinaXiao-Li Zhang - Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou 510632, PR ChinaQiang-Qiang Fu - Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou 510632, PR ChinaJun-Hui Zou - Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou 510632, PR ChinaYueHe Lin - Pacific Northwest National Laboratory, Richland, WA 99352, USA
- Publication Details
- Analytica chimica acta, Vol.745, pp.99-105
- Academic Unit
- Mechanical and Materials Engineering, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546632301842
- Language
- English
- Resource Type
- Journal article