Journal article
Colony stimulating factor 1 is an extrinsic stimulator of mouse spermatogonial stem cell self-renewal
Development (Cambridge), Vol.136(7), pp.1191-1199
04/01/2009
Handle:
https://hdl.handle.net/2376/101120
PMCID: PMC2685936
PMID: 19270176
Abstract
Self-renewal and differentiation of spermatogonial stem cells (SSCs)
provide the foundation for testis homeostasis, yet mechanisms that control
their functions in mammals are poorly defined. We used microarray transcript
profiling to identify specific genes whose expressions are augmented in the
SSC-enriched Thy1
+
germ cell fraction of mouse pup testes.
Comparisons of gene expression in the Thy1
+
germ cell fraction with
the Thy1-depleted testis cell population identified 202 genes that are
expressed 10-fold or higher in Thy1
+
cells. This database provided
a mining tool to investigate specific characteristics of SSCs and identify
novel mechanisms that potentially influence their functions. These analyses
revealed that colony stimulating factor 1 receptor (
Csf1r
) gene
expression is enriched in Thy1
+
germ cells. Addition of recombinant
colony stimulating factor 1 (Csf1), the specific ligand for Csf1r, to culture
media significantly enhanced the self-renewal of SSCs in heterogeneous
Thy1
+
spermatogonial cultures over a 63-day period without
affecting total germ cell expansion. In vivo, expression of Csf1 in both
pre-pubertal and adult testes was localized to clusters of Leydig cells and
select peritubular myoid cells. Collectively, these results identify Csf1 as
an extrinsic stimulator of SSC self-renewal and implicate Leydig and myoid
cells as contributors of the testicular stem cell niche in mammals.
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Details
- Title
- Colony stimulating factor 1 is an extrinsic stimulator of mouse spermatogonial stem cell self-renewal
- Creators
- Jon M Oatley - Department of Dairy and Animal Sciences, Center for Reproductive Biology and Health, College of Agricultural Sciences, Pennsylvania State University, University Park, PA 16802, USAMelissa J Oatley - Department of Dairy and Animal Sciences, Center for Reproductive Biology and Health, College of Agricultural Sciences, Pennsylvania State University, University Park, PA 16802, USAMary R Avarbock - Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USAJohn W Tobias - Penn Bioinformatics Core, University of Pennsylvania, Philadelphia, PA 19104, USARalph L Brinster - Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
- Publication Details
- Development (Cambridge), Vol.136(7), pp.1191-1199
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Company of Biologists
- Identifiers
- 99900546569001842
- Language
- English
- Resource Type
- Journal article