Journal article
Colorimetric and Chemiluminescent Dual-Readout Immunochromatographic Assay for Detection of Pesticide Residues Utilizing g-C3N4/BiFeO3 Nanocomposites with Peroxidase-Like Activity
Biosensors & bioelectronics, Vol.106, pp.43-49
01/31/2018
PMID: 29414087
Abstract
Graphitic carbon nitride/bismuth ferrite nanocomposites (g-C 3 N 4 /BiFeO 3 NCs) were synthesized by a facile one step sol-gel combustion method and employed as a peroxidase-like catalyst. Based on the catalytical activity on the luminol-H 2 O 2 reaction, the nanocomposites were utilized as a colorimetric/chemiluminescent dual-readout immunochromatographic assay (ICA) for the multiplexed detection of pesticide residues by utilizing chlorpyrifos and carbaryl as the model analytes. In the proposed protocol, chlorpyrifos antibody and carbaryl antibody were tagged to g-C 3 N 4 /BiFeO 3 NCs for developing the spatially-resolved multianalyte ICA. After two competitive immunoreactions completed on the ICA test strip, the tracer antibodies were captured by the immobilized antigens on two test lines. The accumulation of g-C 3 N 4 /BiFeO 3 NCs led to the appearance of brown color, which were observed as a colorimetric and semi-quantitative signal. Furthermore, the g-C 3 N 4 /BiFeO 3 NCs-driven generation of CL signal was collected as a sensitively quantitative signal after initiating the luminol-H 2 O 2 reaction on the test lines. Under the optimal conditions, the limits of detection of chlorpyrifos and carbaryl were both 0.033 ng/mL. The dual-readout ICA was successfully used to detect chlorpyrifos and carbaryl spiked in environmental water and traditional Chinese medicine samples with acceptable recovery values of 80%–119% and 90%–118%. Due to many advantages including low cost, time efficiency, high sensitivity and good portability, the novel ICA showed great potential in many areas such as drug safety, environmental monitoring and clinical diagnosis.
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Details
- Title
- Colorimetric and Chemiluminescent Dual-Readout Immunochromatographic Assay for Detection of Pesticide Residues Utilizing g-C3N4/BiFeO3 Nanocomposites with Peroxidase-Like Activity
- Creators
- Hui Ouyang - Washington State UniversityXinman Tu - Washington State UniversityZhifeng Fu - Southwest UniversityWenwen Wang - Southwest UniversityShaofang Fu - Washington State UniversityChengzhou Zhu - Washington State UniversityDan Du - Washington State UniversityYuehe Lin - Washington State University
- Publication Details
- Biosensors & bioelectronics, Vol.106, pp.43-49
- Academic Unit
- School of Mechanical and Materials Engineering
- Identifiers
- 99901227644901842
- Language
- English
- Resource Type
- Journal article