Journal article
Combinatorial engineering of Yarrowia lipolytica as a promising cell biorefinery platform for the de novo production of multi-purpose long chain dicarboxylic acids
Fermentation (Basel), Vol.3(3), p.40
2017
Handle:
https://hdl.handle.net/2376/111479
Abstract
This proof-of-concept study establishes Yarrowia lipolytica (Y. lipolytica) as a whole cell factory for the de novo production of long chain dicarboxylic acid (LCDCA-16 and 18) using glycerol as the sole source of carbon. Modification of the fatty acid metabolism pathway enabled creating a pool of fatty acids in a β-oxidation deficient strain. We then selectively upregulated the native fatty acid ω-oxidation pathway for the enhanced terminal oxidation of the endogenous fatty acid precursors. Nitrogen-limiting conditions and leucine supplementation were employed to induce fatty acid biosynthesis in an engineered Leu− modified strain. Our genetic engineering strategy allowed a minimum production of 330 mg/L LCDCAs in shake flask. Scale up to a 1-L bioreactor increased the titer to 3.49 g/L. Our engineered yeast also produced citric acid as a major by-product at a titer of 39.2 g/L. These results provide basis for developing Y. lipolytica as a safe biorefinery platform for the sustainable production of high-value LCDCAs from non-oily feedstock.
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Details
- Title
- Combinatorial engineering of Yarrowia lipolytica as a promising cell biorefinery platform for the de novo production of multi-purpose long chain dicarboxylic acids
- Creators
- Ali Abghari - Washington State UniversityCatherine Madzak - Génie et Microbiologie des Procédés AlimentairesShulin Chen - Washington State University
- Publication Details
- Fermentation (Basel), Vol.3(3), p.40
- Publisher
- MDPI
- Identifiers
- 99900582332301842
- Language
- English
- Resource Type
- Journal article