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Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients3.24 MB
CC BY V4.0, Open Access
Journal article
Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients
Oncotarget, Vol.7(37), pp.59877-59891
09/13/2016
PMCID: PMC5312355
PMID: 27494883
Abstract
Circulating tumor cells (CTCs) have emerged as promising tools for noninvasive cancer detection and prognosis. Most conventional approaches for capturing CTCs use an EpCAM-based enrichment strategy, which does not work well in cancers that show low or no expression of EpCAM, such as renal cell carcinoma (RCC). In this study, we developed a new set of cell surface markers including CA9 and CD147 as alternative CTC-capture antigens specifically designed for RCC patients. We showed that the expression of both CA9 and CD147 was prevalent in a RCC patient cohort (n=70) by immunohistochemical analysis, with both molecules in combination covering 97.1% of cases. The NanoVelcro platform combined with CA9-/CD147-capture antibodies demonstrated significantly higher efficiency for capturing both CTC-mimicking renal cancer cells and RCC CTCs in peripheral blood, compared to the conventional EpCAM-based method. Using immunofluorescence cytological validation at the single-cell level, we were able to identify bona fide CTCs in RCC patient blood following the well-accepted criteria in our CTC-capture system. We further demonstrated a significant association of CTC numbers as well as the CTC expression status of Vimentin, a mesenchymal marker, with disease progression, including pathologic features and clinical staging. These results provide new insights into developing novel, effective targets/approaches for capturing CTCs, making CTCs a valuable tool for improved cancer detection, prognosis and treatment in RCC.
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Details
- Title
- Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients
- Creators
- Shijie Liu - Fourth Military Medical UniversityZuhong Tian - Fourth Military Medical UniversityLei Zhang - Department of Epidemiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, ChinaShuang Hou - Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, California Nanosystems Institute, University of California, Los Angeles, CA 90095, USASijun Hu - Fourth Military Medical UniversityJunshen Wu - Fourth Military Medical UniversityYuming Jing - Fourth Military Medical UniversityHuimin Sun - Fourth Military Medical UniversityFei Yu - Fourth Military Medical UniversityLibo Zhao - Chinese Academy of SciencesRuoxiang Wang - Cedars-Sinai Medical CenterHsian-Rong Tseng - Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, California Nanosystems Institute, University of California, Los Angeles, CA 90095, USAHaiyen E Zhau - Cedars-Sinai Medical CenterLeland W K Chung - Cedars-Sinai Medical CenterKaichun Wu - Fourth Military Medical UniversityHao Wang - Center for NanoScienceJason Boyang Wu - Washington State University, Pharmaceutical Sciences, Department ofYongzhan Nie - Fourth Military Medical UniversityChen Shao - Fourth Military Medical University
- Publication Details
- Oncotarget, Vol.7(37), pp.59877-59891
- Academic Unit
- Pharmacy and Pharmaceutical Sciences, College of
- Grant note
- P01 CA098912 / NCI NIH HHS R44 CA180482 / NCI NIH HHS R33 CA174562 / NCI NIH HHS U01 CA198900 / NCI NIH HHS
- Identifiers
- 99900620489501842
- Language
- English
- Resource Type
- Journal article