Journal article
Comparative Bioinformatics Analysis of Transcription Factor Genes Indicates Conservation of Key Regulatory Domains among Babesia bovis, Babesia microti, and Theileria equi
PLoS neglected tropical diseases, Vol.10(11), pp.e0004983-e0004983
11/2016
Handle:
https://hdl.handle.net/2376/106232
PMCID: PMC5104403
PMID: 27832060
Abstract
Apicomplexa tick-borne hemoparasites, including Babesia bovis, Babesia microti, and Theileria equi are responsible for bovine and human babesiosis and equine theileriosis, respectively. These parasites of vast medical, epidemiological, and economic impact have complex life cycles in their vertebrate and tick hosts. Large gaps in knowledge concerning the mechanisms used by these parasites for gene regulation remain. Regulatory genes coding for DNA binding proteins such as members of the Api-AP2, HMG, and Myb families are known to play crucial roles as transcription factors. Although the repertoire of Api-AP2 has been defined and a HMG gene was previously identified in the B. bovis genome, these regulatory genes have not been described in detail in B. microti and T. equi. In this study, comparative bioinformatics was used to: (i) identify and map genes encoding for these transcription factors among three parasites' genomes; (ii) identify a previously unreported HMG gene in B. microti; (iii) define a repertoire of eight conserved Myb genes; and (iv) identify AP2 correlates among B. bovis and the better-studied Plasmodium parasites. Searching the available transcriptome of B. bovis defined patterns of transcription of these three gene families in B. bovis erythrocyte stage parasites. Sequence comparisons show conservation of functional domains and general architecture in the AP2, Myb, and HMG proteins, which may be significant for the regulation of common critical parasite life cycle transitions in B. bovis, B. microti, and T. equi. A detailed understanding of the role of gene families encoding DNA binding proteins will provide new tools for unraveling regulatory mechanisms involved in B. bovis, B. microti, and T. equi life cycles and environmental adaptive responses and potentially contributes to the development of novel convergent strategies for improved control of babesiosis and equine piroplasmosis.
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Details
- Title
- Comparative Bioinformatics Analysis of Transcription Factor Genes Indicates Conservation of Key Regulatory Domains among Babesia bovis, Babesia microti, and Theileria equi
- Creators
- Heba F Alzan - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of AmericaDonald P Knowles - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of AmericaCarlos E Suarez - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America
- Publication Details
- PLoS neglected tropical diseases, Vol.10(11), pp.e0004983-e0004983
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900546777801842
- Language
- English
- Resource Type
- Journal article