Journal article
Comparative gene expression by WC1+ γδ and CD4+ αβ T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell‐associated genes by WC1+ γδ T cells
Journal of leukocyte biology, Vol.80(4), pp.939-952
10/2006
Handle:
https://hdl.handle.net/2376/108926
PMID: 17005908
Abstract
The functions of γδ T cells are enigmatic, and these cells are often considered as evolutionary remnants of well‐characterized αβ T cells. However, their conservation throughout evolution suggests that γδ T cells are biologically unique. In ruminants, γδ T cells expressing the workshop cluster 1 (WC1) scavenger receptor comprise a large proportion of circulating lymphocytes, suggesting these cells are biologically relevant and functionally different from αβ T cells. In fact, bovine WC1+ γδ T cells can act as APC for αβ T cells, indicating they may express genes encoding proteins associated with innate immunity. The present study was designed to compare immune function gene expression profiles of clonal populations of WC1+ γδ and CD4+ αβ T cells derived from the same animal, which respond to major surface protein 2 (MSP2) of the intraerythrocytic rickettsial pathogen of cattle, Anaplasma marginale. Gene expression profiles of activated T cell clones were compared using a microarray format, and differential gene expression was confirmed by real‐time RT‐PCR and protein analyses. We demonstrate that although MSP2‐specific αβ and γδ T cell clones express many of the same genes, γδ T cell clones express high levels of genes associated with myeloid cells, including chemokines CCL2, CXCL1, CXCL2, CXCL6, and surface receptors CD68, CD11b, macrophage scavenger receptor 1, macrophage mannose receptor, and galectin‐3. It is important that many of these genes were also expressed at higher levels in polyclonal WC1+ γδ T cells when compared with CD4+ αβ T cells selected from peripheral blood.
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Details
- Title
- Comparative gene expression by WC1+ γδ and CD4+ αβ T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell‐associated genes by WC1+ γδ T cells
- Creators
- Kevin K LahmersJodi F HedgesMark A JutilaMingqi DengMitchell S AbrahamsenWendy C Brown
- Publication Details
- Journal of leukocyte biology, Vol.80(4), pp.939-952
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Number of pages
- 14
- Grant note
- U.S. Department of Agriculture National Research Initiative Competitive Grants Program (00‐52100‐9612) National Institute of Allergy and Infectious Diseases (K08 AI53594) National Institutes of Health
- Identifiers
- 99900547241401842
- Language
- English
- Resource Type
- Journal article