Journal article
Comparison of SHF and SSF of wet exploded corn stover and loblolly pine using in-house enzymes produced from T. reesei RUT C30 and A. saccharolyticus
SpringerPlus, Vol.3(1), pp.1-13
12/2014
Handle:
https://hdl.handle.net/2376/101618
PMCID: PMC4176844
PMID: 25279308
Abstract
The aim of the present study was to compare bioethanol production from wet exploded corn stover (WECS) and loblolly pine (WELP) hydrolyzed with in-house and commercial enzymes and fermented separately (SHF) and simultaneously (SSF). In-house enzymes produced from
Trichoderma reesei
, RUT-C30 and a novel fungal strain,
Aspergillus saccharolyticus
were loaded as 5 and 15 FPU/g glucan and supplemented with 10 and 30 CBU/g glucan, respectively. For hydrolysis and fermentation, slurries of WECS and WELP at 5 and 10% (w/w) solids loading (SL) were utilized.
Saccharomyces cerevisae
was used for ethanol fermentation at 33°C. Maximally, 15.6 g/L and 13.4 g/L (corresponding to theoretical ethanol yield of 76% and 67%, respectively) were achieved in SSF process from WECS and WELP, respectively at 5% SL and 15 FPU/g glucan loading of in-house enzymes. Ethanol concentrations in all cases were higher for SSF compared to SHF under same conditions. A cross comparison of SSF with commercial enzymes (Celluclast 1.5 L + Novozym 188) showed highest ethanol concentration of 17.3 g/L and 15.4 g/L (corresponding to theoretical ethanol yield of 84% and 77%, respectively) from WECS and WELP, respectively at 5% SL and 15 FPU/g glucan. These findings demonstrated that in-house enzymes were comparable to commercial enzymes as these fungi produced other lignocellulolytic enzymes beyond cellulase and hence enhanced the overall enzyme activity.
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Details
- Title
- Comparison of SHF and SSF of wet exploded corn stover and loblolly pine using in-house enzymes produced from T. reesei RUT C30 and A. saccharolyticus
- Creators
- Vandana Rana - Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University, 2710 Crimson Way, Richland, WA 99354-1671 USAAnahita D Eckard - Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University, 2710 Crimson Way, Richland, WA 99354-1671 USABirgitte K Ahring - Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University, 2710 Crimson Way, Richland, WA 99354-1671 USA
- Publication Details
- SpringerPlus, Vol.3(1), pp.1-13
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Springer International Publishing; Cham
- Identifiers
- 99900546679301842
- Language
- English
- Resource Type
- Journal article