Comparison of Salmonella enterica serotype Infantis isolates from a veterinary teaching hospital

M Dunowska; P S Morley; J L Traub-Dargatz; M A Davis; G Patterson; J G Frye; D R Hyatt; D A Dargatz

doi:10.1111/j.1365-2672.2006.03198.x

Back

Comparison of Salmonella enterica serotype Infantis isolates from a veterinary teaching hospital

Journal article

Peer reviewed

Comparison of Salmonella enterica serotype Infantis isolates from a veterinary teaching hospital

M Dunowska, P S Morley, J L Traub-Dargatz, M A Davis, G Patterson, J G Frye, D R Hyatt and D A Dargatz

Journal of applied microbiology, Vol.102(6), pp.1527-1536

06/2007

DOI: https://doi.org/10.1111/j.1365-2672.2006.03198.x

Handle:

https://hdl.handle.net/2376/115361

PMID: 17578417

Abstract

Integrons - genetics

Humans

Drug Resistance, Bacterial

Salmonella enterica - genetics

Hospitals, Animal

Polymerase Chain Reaction - methods

Salmonella enterica - drug effects

Phenotype

Animals

DNA, Bacterial - genetics

Disease Outbreaks

Cross Infection - microbiology

Electrophoresis, Gel, Pulsed-Field - methods

Salmonella Infections, Animal - microbiology

Salmonella enterica - isolation & purification

Salmonella Infections - microbiology

Anti-Bacterial Agents - pharmacology

Retrospective Studies

Microbial Sensitivity Tests - methods

To compare Salmonella enterica serotype Infantis isolates obtained from patients or the environment of a veterinary teaching hospital over a period of 9 years following a nosocomial outbreak to determine whether isolates were epidemiologically related or represented unrelated introductions into the hospital environment. Fifty-six S. Infantis isolates were compared based on their phenotypic (antimicrobial drug [AMD] susceptibility pattern) and genotypic (pulsed-field gel electrophoresis [PFGE] pattern and presence of integrons) characteristics. Epidemiologically unrelated S. Infantis isolates clustered separately from all but two of the hospital isolates, and several isolates from different years and various sources were indistinguishable from each other in cluster analysis of two-enzyme PFGE results. A high percentage of isolates (80.3%) were resistant to at least one AMD, with 67.8% showing resistance to >5 AMD. The majority (74.1%) of isolates tested contained type 1 integrons. Results strongly suggest that there was nosocomial transmission of S. Infantis during the initial outbreak, and that contamination arising from this outbreak persisted across years despite rigorous hygiene and biosecurity precautions and may have led to subsequent nosocomial infections. Evidence of persistence and transmission of Salmonella clones across years, even in the face of rigorous preventive measures, has important implications for other facilities that have experienced outbreaks of Salmonella infections.

Metrics

6 Record Views

Details

Title: Comparison of Salmonella enterica serotype Infantis isolates from a veterinary teaching hospital
Creators: M Dunowska - Animal Population Health Institute, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
P S Morley
J L Traub-Dargatz
M A Davis
G Patterson
J G Frye
D R Hyatt
D A Dargatz
Publication Details: Journal of applied microbiology, Vol.102(6), pp.1527-1536
Academic Unit: Paul G. Allen School for Global Animal Health
Publisher: England
Grant note: N01-AI-30055 / NIAID NIH HHS
Identifiers: 99900547412801842
Language: English
Resource Type: Journal article

Comparison of Salmonella enterica serotype Infantis isolates from a veterinary teaching hospital

Related links

Abstract

Metrics

Details