Journal article
Comparison of isothiocyanate metabolite levels and histone deacetylase activity in human subjects consuming broccoli sprouts or broccoli supplement
Journal of agricultural and food chemistry, Vol.59(20), pp.10955-10963
10/26/2011
Handle:
https://hdl.handle.net/2376/106630
PMCID: PMC3201700
PMID: 21928849
Abstract
Increased consumption of cruciferous vegetables such as broccoli may reduce the risk of various cancers. Myrosinase is required to convert dietary glucosinolates from broccoli into bioactive isothiocyanates. We evaluated isothiocyanate excretion profiles in healthy subjects who consumed broccoli sprouts or broccoli supplement (no myrosinase) with equivalent glucosinolate content. Urinary metabolites of two major isothiocyanates, sulforaphane and erucin, were measured by liquid chromatography coupled with tandem mass spectrometry. Peak excretion of sulforaphane and erucin was higher and occurred sooner in subjects who consumed broccoli sprouts compared to subjects who consumed the supplement. A subject-dependent shift in the ratio of urinary sulforaphane to erucin metabolites was observed in both groups indicating conversion of sulforaphane to erucin. Lower histone deacetylase activity was observed in the peripheral blood mononuclear cells only in subjects consuming sprouts. In conclusion, fresh broccoli sprouts differ from broccoli supplements in regards to excretion of isothiocyanates and bioactivity in human subjects.
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Details
- Title
- Comparison of isothiocyanate metabolite levels and histone deacetylase activity in human subjects consuming broccoli sprouts or broccoli supplement
- Creators
- John D Clarke - Department of Nutrition and Exercise Sciences, Oregon State University, Corvallis, OR 97331Ken Riedl - The Ohio State University, Columbus, OH 43210Deborah Bella - Department of Nutrition and Exercise Sciences, Oregon State University, Corvallis, OR 97331Steven J Schwartz - The Ohio State University, Columbus, OH 43210Jan F Stevens - Linus Pauling Institute, Oregon State University, Corvallis, OR 97331Emily Ho - Department of Nutrition and Exercise Sciences, Oregon State University, Corvallis, OR 97331
- Publication Details
- Journal of agricultural and food chemistry, Vol.59(20), pp.10955-10963
- Academic Unit
- Pharmaceutical Sciences, Department of
- Grant note
- P01 CA090890-07 || CA / National Cancer Institute : NCI R01 CA122906-03S2 || CA / National Cancer Institute : NCI
- Identifiers
- 99900546803901842
- Language
- English
- Resource Type
- Journal article