Journal article
Comparison of multiple genes of spring viremia of carp viruses isolated in the United States
Virus genes, Vol.35(1), pp.87-95
08/2007
Handle:
https://hdl.handle.net/2376/108192
PMID: 17048110
Abstract
Five spring viremia of carp viruses (SVCV), Rhabdovirus carpio, were isolated in the United States (US) between 2002 and 2004. Single tube reverse transcription-polymerase chain reaction (RT-PCR) was used to generate overlapping cDNA fragments from the US isolates of SVCV. Multiple pairs of specific primers were designed to amplify a portion of the phosphoprotein gene, the matrix gene, and the glycoprotein gene of SVCV genogroup Id (corresponding to nucleotides 2174-4942 of GenBank accession NC_002803). Sequences were proofread and aligned to generate a consensus sequence for each isolate. Phylogenetic analysis of the 2705 nucleotide consensus sequence revealed that all five US isolates belong to SVCV genogroup Ia, Asian origin isolates, and a PCR primer binding site unique to SVCV genogroup Ia was identified.
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Details
- Title
- Comparison of multiple genes of spring viremia of carp viruses isolated in the United States
- Creators
- Janet V Warg - Diagnostic Virology Laboratory, National Veterinary Services Laboratories, VS-APHIS-USDA, Ames, IA 50010, USA. Janet.V.Warg@aphis.usda.govAudrey L DikkeboomAndrew E GoodwinKevin SnekvikJohn Whitney
- Publication Details
- Virus genes, Vol.35(1), pp.87-95
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900547224401842
- Language
- English
- Resource Type
- Journal article