Journal article
Complete Genome Sequence of the Marine, Chemolithoautotrophic, Ammonia-Oxidizing Bacterium Nitrosococcus oceani ATCC 19707
Applied and environmental microbiology, Vol.72(9), pp.6299-6315
09/2006
Handle:
https://hdl.handle.net/2376/104208
PMCID: PMC1563620
PMID: 16957257
Abstract
The gammaproteobacterium
Nitrosococcus oceani
(ATCC 19707) is a gram-negative obligate chemolithoautotroph capable of extracting energy and reducing power from the oxidation of ammonia to nitrite. Sequencing and annotation of the genome revealed a single circular chromosome (3,481,691 bp; G+C content of 50.4%) and a plasmid (40,420 bp) that contain 3,052 and 41 candidate protein-encoding genes, respectively. The genes encoding proteins necessary for the function of known modes of lithotrophy and autotrophy were identified. Contrary to betaproteobacterial nitrifier genomes, the
N. oceani
genome contained two complete
rrn
operons. In contrast, only one copy of the genes needed to synthesize functional ammonia monooxygenase and hydroxylamine oxidoreductase, as well as the proteins that relay the extracted electrons to a terminal electron acceptor, were identified. The
N. oceani
genome contained genes for 13 complete two-component systems. The genome also contained all the genes needed to reconstruct complete central pathways, the tricarboxylic acid cycle, and the Embden-Meyerhof-Parnass and pentose phosphate pathways. The
N. oceani
genome contains the genes required to store and utilize energy from glycogen inclusion bodies and sucrose. Polyphosphate and pyrophosphate appear to be integrated in this bacterium's energy metabolism, stress tolerance, and ability to assimilate carbon via gluconeogenesis. One set of genes for type I ribulose-1,5-bisphosphate carboxylase/oxygenase was identified, while genes necessary for methanotrophy and for carboxysome formation were not identified. The
N. oceani
genome contains two copies each of the genes or operons necessary to assemble functional complexes I and IV as well as ATP synthase (one H
+
-dependent F
0
F
1
type, one Na
+
-dependent V type).
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Details
- Title
- Complete Genome Sequence of the Marine, Chemolithoautotrophic, Ammonia-Oxidizing Bacterium Nitrosococcus oceani ATCC 19707
- Creators
- Martin G Klotz - University of Louisville, Louisville, Kentucky 40292Daniel J Arp - University of Louisville, Louisville, Kentucky 40292Patrick S. G Chain - University of Louisville, Louisville, Kentucky 40292Amal F El-Sheikh - University of Louisville, Louisville, Kentucky 40292Loren J Hauser - University of Louisville, Louisville, Kentucky 40292Norman G Hommes - University of Louisville, Louisville, Kentucky 40292Frank W Larimer - University of Louisville, Louisville, Kentucky 40292Stephanie A Malfatti - University of Louisville, Louisville, Kentucky 40292Jeanette M Norton - University of Louisville, Louisville, Kentucky 40292Amisha T Poret-Peterson - University of Louisville, Louisville, Kentucky 40292Lisa M Vergez - University of Louisville, Louisville, Kentucky 40292Bess B Ward - University of Louisville, Louisville, Kentucky 40292
- Publication Details
- Applied and environmental microbiology, Vol.72(9), pp.6299-6315
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547093801842
- Language
- English
- Resource Type
- Journal article