Journal article
Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins
Proceedings of the National Academy of Sciences - PNAS, Vol.102(3), pp.844-849
01/18/2005
Handle:
https://hdl.handle.net/2376/111022
PMCID: PMC545514
PMID: 15618402
Abstract
The rickettsia
Anaplasma marginale
is the most prevalent tick-borne livestock pathogen worldwide and is a severe constraint to animal health.
A. marginale
establishes lifelong persistence in infected ruminants and these animals serve as a reservoir for ticks to acquire and transmit the pathogen. Within the mammalian host,
A. marginale
generates antigenic variants by changing a surface coat composed of numerous proteins. By sequencing and annotating the complete 1,197,687-bp genome of the St. Maries strain of
A. marginale
, we show that this surface coat is dominated by two families containing immunodominant proteins: the
msp
2 superfamily and the
msp
1 superfamily. Of the 949 annotated coding sequences, just 62 are predicted to be outer membrane proteins, and of these, 49 belong to one of these two superfamilies. The genome contains unusual functional pseudogenes that belong to the
msp
2 superfamily and play an integral role in surface coat antigenic variation, and are thus distinctly different from pseudogenes described as byproducts of reductive evolution in other
Rickettsiales
.
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Details
- Title
- Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins
- Creators
- Kelly A Brayton - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washingston State University, Pullman, WA 99164-7040; andLowell S Kappmeyer - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washingston State University, Pullman, WA 99164-7040; andDavid R Herndon - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washingston State University, Pullman, WA 99164-7040; andMichael J Dark - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washingston State University, Pullman, WA 99164-7040; andDavid L Tibbals - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washingston State University, Pullman, WA 99164-7040; andGuy H Palmer - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washingston State University, Pullman, WA 99164-7040; andTravis C McGuire - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washingston State University, Pullman, WA 99164-7040; andDonald P Knowles - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washingston State University, Pullman, WA 99164-7040; and
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.102(3), pp.844-849
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- National Academy of Sciences
- Identifiers
- 99900548460401842
- Language
- English
- Resource Type
- Journal article