Journal article
Complex Assembly and Metabolic Profiling of Arabidopsis thaliana Plants Overexpressing Vitamin B6 Biosynthesis Proteins
Molecular plant, Vol.3(5), pp.890-903
09/2010
Handle:
https://hdl.handle.net/2376/106120
PMID: 20675613
Abstract
In plants, vitamin B6 biosynthesis requires the activity of PDX1 and PDX2 proteins. Arabidopsis thaliana encodes for three PDX1 proteins, named PDX1.1, 1.2, and 1.3, but only one PDX2. Here, we show in planta complex assembly of PDX proteins, based on split-YFP and FPLC assays, and can demonstrate their presence in higher complexes of around 750 kDa. Metabolic profiling of plants ectopically expressing the different PDX proteins indicates a negative influence of PDX1.2 on vitamin B6 biosynthesis and a correlation between aberrant vitamin B6 content, PDX1 gene expression, and light sensitivity specifically for PDX1.3. These findings provide first insights into in planta vitamin B6 synthase complex assembly and new information on how the different PDX proteins affect plant metabolism.
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Details
- Title
- Complex Assembly and Metabolic Profiling of Arabidopsis thaliana Plants Overexpressing Vitamin B6 Biosynthesis Proteins
- Creators
- Jan Erik Leuendorf - Angewandte Genetik, Freie Universität Berlin, 14195 Berlin, GermanySonia Osorio - Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm 14476, GermanyAgnieszka Szewczyk - Pharmaceutical Faculty of the Collegium Medicum, Jagiellonian University, Krakow, PolandAlisdair R Fernie - Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm 14476, GermanyHanjo Hellmann - School of Biological Sciences, Washington State University, Pullman, USA
- Publication Details
- Molecular plant, Vol.3(5), pp.890-903
- Academic Unit
- Biological Sciences, School of
- Publisher
- Elsevier Inc
- Identifiers
- 99900546741501842
- Language
- English
- Resource Type
- Journal article