Journal article
Computer-Assisted Rational Modifications to Improve the Thermostability of β-Glucosidase from Penicillium piceum H16
Bioenergy research, Vol.8(3), pp.1384-1390
09/2015
Handle:
https://hdl.handle.net/2376/118388
Abstract
β-Glucosidase plays key role in the saccharification of cellulosic biomass. The use of β-glucosidase would benefit from improvements in its thermostability in some industrial applications. In this work, two rational computer-aided strategies were devised to enhance the thermostability of a highly active β-glucosidase from Penicillium piceum H16. Single-point mutants were proposed based on the proline theory. The effects of the mutation on protein stability were further predicted using molecular dynamics (MD) simulations. The optimal single mutant, G305P, was found to provide a 20.0 % improvement in thermostability. Then computer-assisted virtual saturated mutation was first used to select the optimal triple mutations which would further increase the thermostability of β-glucosidase by the Calculate Mutation Energy/Stability module of Discovery Studio (DS) 4.0. The triple mutant, S507F/Q512W/S514W, provided a 46.3 % improvement in thermostability. A detailed structural analysis showed that the additional hydrophobic interactions played decisive roles in the improvement in thermostability, and the use of mutants with enhanced stability may benefit formulation of more effective enzyme cocktails for lignocellulosic bioconversion.
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Details
- Title
- Computer-Assisted Rational Modifications to Improve the Thermostability of β-Glucosidase from Penicillium piceum H16
- Creators
- Zhiyou Zong - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin ChinaLe Gao - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin ChinaWensheng Cai - College of Chemistry Nankai University Tianjin ChinaLiang Yu - Department of Biological Systems Engineering Washington State University Pullman WA 99164 USAChao Cui - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin ChinaShulin Chen - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin ChinaDongyuan Zhang - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin China
- Contributors
- Steven C Slater (Editor)Blake A Simmons (Editor)Shawn Kaeppler (Editor)Brian H Davison (Editor)Christos Maravelius (Editor)Intawat Nookeaw (Editor)
- Publication Details
- Bioenergy research, Vol.8(3), pp.1384-1390
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Springer US
- Identifiers
- 99900583056801842
- Language
- English
- Resource Type
- Journal article