Journal article
Conformational Dependence of Anaplasma marginale Major Surface Protein 5 Surface-Exposed B-Cell Epitopes
Infection and immunity, Vol.66(6), pp.2619-2624
06/1998
Handle:
https://hdl.handle.net/2376/101889
PMCID: PMC108247
PMID: 9596725
Abstract
The
Anaplasma marginale
outer membrane is composed of immunogenic major surface proteins (MSPs) linked both covalently and noncovalently in multimeric complexes (M. C. Vidotto, T. C. McGuire, T. F. McElwain, G. H. Palmer, and D. P. Knowles, Infect. Immun. 62:2940–2946). Consequently, effective induction of antibody against surface-exposed MSP epitopes has been postulated to require maintenance of MSP secondary through quatenary structures. Using MSP5 as a model and the approach of epitope mapping with recombinant expressed full-length and truncated proteins, we demonstrated that the immunodominant surface epitope bound by monoclonal antibody (MAb) ANAF16C1 required disparate amino- and carboxy-terminal regions of MSP5, indicating the conformational dependence of this epitope. The required amino-terminal MSP5 region included the cysteines involved in intramolecular disulfide bonding. The dependence of the immunodominant epitope on disulfide bonding was confirmed by loss of MAb ANAF16C1 binding to MSP5 following disulfide bond reduction and covalent modification of the reduced sulfhydryl groups. The recognition of the MSP5 immunodominant epitope by antibody induced by protective immunization with
A. marginale
outer membranes was also conformationally dependent, as shown by the loss of epitope binding following serum adsorption with native but not reduced and denatured
A. marginale
. Importantly, the antibody response to all immunodominant MSP5 surface epitopes was restricted to conformationally dependent epitopes, since the binding of polyclonal anti-MSP5 antibody to the
A. marginale
surface could be blocked by adsorption with native but not denatured and reduced MSP5. These results confirm the importance of the secondary and tertiary structures of MSP epitopes as immune system targets and support the testing of immunogens which maintain the required conformation.
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Details
- Title
- Conformational Dependence of Anaplasma marginale Major Surface Protein 5 Surface-Exposed B-Cell Epitopes
- Creators
- Devere Munodzana - Laboratory Diagnostics and Research Branch, Central Veterinary Laboratory, Harare, ZimbabweTerry F McElwain - Laboratory Diagnostics and Research Branch, Central Veterinary Laboratory, Harare, ZimbabweDonald P Knowles - Laboratory Diagnostics and Research Branch, Central Veterinary Laboratory, Harare, ZimbabweGuy H Palmer - Laboratory Diagnostics and Research Branch, Central Veterinary Laboratory, Harare, Zimbabwe
- Publication Details
- Infection and immunity, Vol.66(6), pp.2619-2624
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546669301842
- Language
- English
- Resource Type
- Journal article