Journal article
Conservation of Babesia bovis Small Heat Shock Protein (Hsp20) among Strains and Definition of T Helper Cell Epitopes Recognized by Cattle with Diverse Major Histocompatibility Complex Class II Haplotypes
Infection and immunity, Vol.72(2), pp.1096-1106
02/2004
Handle:
https://hdl.handle.net/2376/111345
PMCID: PMC321645
PMID: 14742557
Abstract
Babesia bovis
small heat shock protein (Hsp20) is recognized by CD4
+
T lymphocytes from cattle that have recovered from infection and are immune to challenge. This candidate vaccine antigen is related to a protective antigen of
Toxoplasma gondii
, Hsp30/bag1, and both are members of the α-crystallin family of proteins that can serve as molecular chaperones. In the present study, immunofluorescence microscopy determined that Hsp20 is expressed intracellularly in all merozoites. Importantly, Hsp20 is also expressed by tick larval stages, including sporozoites, so that natural tick-transmitted infection could boost a vaccine-induced response. The predicted amino acid sequence of Hsp20 from merozoites is completely conserved among different
B. bovis
strains. To define the location of CD4
+
T-cell epitopes for inclusion in a multiepitope peptide or minigene vaccine construct, truncated recombinant Hsp20 proteins and overlapping peptides were tested for their ability to stimulate T cells from immune cattle. Both amino-terminal (amino acids [aa] 1 to 105) and carboxy-terminal (aa 48 to 177) regions were immunogenic for the majority of cattle in the study, stimulating strong proliferation and IFN-γ production. T-cell lines from all individuals with distinct
DRB3
haplotypes responded to aa 11 to 62 of Hsp20, which contained one or more immunodominant epitopes for each animal. One epitope, DEQTGLPIKS (aa 17 to 26), was identified by T-cell clones. The presence of strain-conserved T helper cell epitopes in aa 11 to 62 of the ubiquitously expressed Hsp20 that are presented by major histocompatibility complex class II molecules represented broadly in the Holstein breed supports the inclusion of this region in vaccine constructs to be tested in cattle.
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Details
- Title
- Conservation of Babesia bovis Small Heat Shock Protein (Hsp20) among Strains and Definition of T Helper Cell Epitopes Recognized by Cattle with Diverse Major Histocompatibility Complex Class II Haplotypes
- Creators
- Junzo Norimine - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Juan Mosqueda - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Guy H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Harris A Lewin - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Wendy C Brown - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164
- Publication Details
- Infection and immunity, Vol.72(2), pp.1096-1106
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547542801842
- Language
- English
- Resource Type
- Journal article