Journal article
Conserved recombinant antigens of Anaplasma marginale and Babesia equi for serologic diagnosis
Veterinary parasitology, Vol.57(1), pp.93-96
1995
Handle:
https://hdl.handle.net/2376/104305
PMID: 7597796
Abstract
The competitive inhibition ELISA (CI-ELISA) format overcomes problems associated with antigen purity since the specificity of the CI-ELISA depends solely on the monoclonal antibody (mAb) used. Therefore, the CI-ELISA format is well suited for use with recombinant antigens. Molecular clones expressing a conserved 19 kDa protein of
Anaplasma marginale and a 34 kDa protein of
Babesia equi were derived and characterized. The 19 kDa
A. marginale protein, conserved in all recognized
Anaplasma species, and present in the infected tick salivary gland, was reactive with all bovine immune sera tested. The 34 kDa
B. equi protein contains a protein epitope bound by antibody in equine immune sera from 19 countries. Monoclonal antibodies reactive with these proteins were derived and applied with recombinant copies of the 19 kDa
A. marginale and 34 kDa
B. equi proteins in a CI-ELISA format.
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Details
- Title
- Conserved recombinant antigens of Anaplasma marginale and Babesia equi for serologic diagnosis
- Creators
- D.P Knowles - Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, Pullman, WA 99164-7030, USAL.E Perryman - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAT.F McElwain - Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, WA 99164-7040, USAL.S Kappmeyer - Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, Pullman, WA 99164-7030, USAD Stiller - Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, Pullman, WA 99164-7030, USAG.H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAE.S Visser - Onderstepoort Veterinary Institute, Onderstepoort 0110, South AfricaS.G Hennager - National Veterinary Services Laboratory, Animal and Plant Health Inspection Service, US Department of Agriculture, Ames, IA 50010, USAW.C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAT.C McGuire - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA
- Publication Details
- Veterinary parasitology, Vol.57(1), pp.93-96
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier B.V
- Identifiers
- 99900547075601842
- Language
- English
- Resource Type
- Journal article