Journal article
Constitutive PI3‐K activity is essential for proliferation, but not survival, of Theileria parva‐transformed B cells
Cellular microbiology, Vol.2(4), pp.329-339
08/2000
Handle:
https://hdl.handle.net/2376/116801
PMID: 11207589
Abstract
Theileria is an intracellular parasite that causes lymphoproliferative disorders in cattle, and infection of leucocytes induces a transformed phenotype similar to tumour cells, but the mechanisms by which the parasite induces this phenotype are not understood. Here, we show that infected B lymphocytes display constitutive phosphoinositide 3‐kinase (PI3‐K) activity, which appears to be necessary for proliferation, but not survival. Importantly, we demonstrate that one mechanism by which PI3‐K mediates the proliferation of infected B lymphocytes is through the induction of a granulocyte–monocyte colony‐stimulating factor (GM‐CSF)‐dependent autocrine loop. PI3‐K induction of GM‐CSF appears to be at the transcriptional level and, consistently, we demonstrate that PI3‐K is also involved in the constitutive induction of AP‐1 and NF‐κB, which characterizes Theileria‐infected leucocytes. Taken together, our results highlight a novel strategy exploited by the intracellular parasite Theileria to induce continued proliferation of its host leucocyte.
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Details
- Title
- Constitutive PI3‐K activity is essential for proliferation, but not survival, of Theileria parva‐transformed B cells
- Creators
- Martin BaumgartnerMarie ChaussepiedMarie‐Françoise MoreauDirk WerlingWilliam C DavisAlphonse GarciaGordon Langsley
- Publication Details
- Cellular microbiology, Vol.2(4), pp.329-339
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Blackwell Science Ltd; Oxford, UK
- Number of pages
- 11
- Identifiers
- 99900548085601842
- Language
- English
- Resource Type
- Journal article