Journal article
Contributions of New Hepatocyte Lineages to Liver Growth, Maintenance, and Regeneration in Mice
Hepatology (Baltimore, Md.), Vol.54(2), pp.655-663
08/2011
Handle:
https://hdl.handle.net/2376/112282
PMCID: PMC3145049
PMID: 21538442
Abstract
The contributions that
de novo
differentiation of new hepatocyte lineages makes to normal liver physiology are unknown. Here a system that uniquely marks cells during a finite period following primary activation of a
serum albumin
gene promoter/enhancer-driven Cre transgene (
albCre
) was used to investigate birthrates of new hepatocyte lineages from Alb-naïve precursors in mice. Elapsed time was measured using a two-color fluorescent marker-gene that converts from expressing tdTomato (tdT, red-fluorescent) to expressing GFP (green-fluorescent) upon exposure to Cre. Accumulation of GFP and decay of tdT each contributed to a regular fluorescence transition, which was calibrated
in vivo
. In normal adults, this system revealed that a steady-state level of 0.076% hepatocytes had differentiated within the previous four days from cell lineages that had never previously expressed
albCre
. As compared to resting adult livers, the relative abundance of these newborn hepatocytes was elevated 3.7-fold in normal growing livers of juveniles and 8.6-fold during liver regeneration following partial hepatectomy in normal adults.
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Details
- Title
- Contributions of New Hepatocyte Lineages to Liver Growth, Maintenance, and Regeneration in Mice
- Creators
- Sonya V Iverson - Department of Immunology and Disease, Montana State University, Bozeman, MT 59718, USAKristin M Comstock - Biology Department, The College of St. Scholastica, Duluth, MN 55811, USAJean A Kundert - Animal Resources Center, Montana State University, Bozeman, MT 59718, USAEdward E Schmidt - Department of Immunology and Disease, Montana State University, Bozeman, MT 59718, USA
- Publication Details
- Hepatology (Baltimore, Md.), Vol.54(2), pp.655-663
- Academic Unit
- UNKNOWN
- Identifiers
- 99900548001501842
- Language
- English
- Resource Type
- Journal article