Journal article
Coordinate Transcriptional Control in the Hyperthermophilic Archaeon Sulfolobus solfataricus
Journal of bacteriology, Vol.181(13), pp.3920-3927
07/1999
Handle:
https://hdl.handle.net/2376/113503
PMCID: PMC93880
PMID: 10383958
Abstract
The existence of a global gene regulatory system in the hyperthermophilic archaeon
Sulfolobus solfataricus
is described. The system is responsive to carbon source quality and acts at the level of transcription to coordinate synthesis of three physically unlinked glycosyl hydrolases implicated in carbohydrate utilization. The specific activities of three enzymes, an α-glucosidase (
malA
), a β-glycosidase (
lacS
), and an α-amylase, were reduced 4-, 20-, and 10-fold, respectively, in response to the addition of supplementary carbon sources to a minimal sucrose medium. Western blot analysis using anti-α-glucosidase and anti-β-glycosidase antibodies indicated that reduced enzyme activities resulted exclusively from decreased enzyme levels. Northern blot analysis of
malA
and
lacS
mRNAs revealed that changes in enzyme abundance arose primarily from reductions in transcript concentrations. Culture conditions precipitating rapid changes in
lacS
gene expression were established to determine the response time of the regulatory system in vivo. Full induction occurred within a single generation whereas full repression occurred more slowly, requiring nearly 38 generations. Since
lacS
mRNA abundance changed much more rapidly in response to a nutrient down shift than to a nutrient up shift, transcript synthesis rather than degradation likely plays a role in the regulatory response.
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Details
- Title
- Coordinate Transcriptional Control in the Hyperthermophilic Archaeon Sulfolobus solfataricus
- Creators
- Cynthia Haseltine - George Beadle Center for Genetics, School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666Rafael Montalvo-Rodriguez - George Beadle Center for Genetics, School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666Elisabetta Bini - George Beadle Center for Genetics, School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666Audrey Carl - George Beadle Center for Genetics, School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666Paul Blum - George Beadle Center for Genetics, School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666
- Publication Details
- Journal of bacteriology, Vol.181(13), pp.3920-3927
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547824401842
- Language
- English
- Resource Type
- Journal article