Journal article
Copy number and haplotype variation at the VRN-A1 and central FR-A2 loci are associated with frost tolerance in hexaploid wheat
Theoretical and applied genetics, Vol.127(5), pp.1183-1197
05/2014
Handle:
https://hdl.handle.net/2376/105953
PMCID: PMC4876961
PMID: 24626953
Abstract
Frost tolerance is critical for wheat survival during cold winters. Natural variation for this trait is mainly associated with allelic differences at the
VERNALIZATION 1
(
VRN1
) and
FROST RESISTANCE 2
(
FR2
) loci.
VRN1
regulates the transition between vegetative and reproductive stages and
FR2
, a locus including several tandemly duplicated
C-REPEAT BINDING FACTOR
(
CBF
) transcription factors, regulates the expression of
Cold regulated
genes. We identified sequence and copy number variation at these two loci among winter and spring wheat varieties and characterized their association with frost tolerance. We identified two
FR-A2
haplotypes – ‘FR-A2-S’ and ‘FR-A2-T’ – distinguished by two insertion/deletions and ten single nucleotide polymorphisms within the
CBF-A12
and
CBF-A15
genes. Increased copy number of
CBF-A14
was frequently associated with the
FR-A2-T
haplotypes and with higher
CBF14
transcript levels in response to cold. Factorial ANOVAs revealed significant interactions between
VRN1
and
FR-A2
for frost tolerance in both winter and spring panels suggesting a crosstalk between vernalization and cold acclimation pathways. The model including these two loci and their interaction explained 32.0 and 20.7% of the variation in frost tolerance in the winter and spring panels, respectively. The interaction was validated in a winter wheat F
4:5
population segregating for both genes. Increased
VRN-A1
copy number was associated with improved frost tolerance among varieties carrying the
FR-A2-T
allele but not among those carrying the
FR-A2-S
allele. These results suggest that selection of varieties carrying the
FR-A2-T
allele and three copies of the recessive
vrn-A1
allele would be a good strategy to improve frost tolerance in wheat.
Metrics
13 Record Views
Details
- Title
- Copy number and haplotype variation at the VRN-A1 and central FR-A2 loci are associated with frost tolerance in hexaploid wheat
- Creators
- Jie Zhu - Crop and Soil Sciences Department, Washington State University, Pullman, WA 99164, USAStephen Pearce - Department of Plant Sciences, University of California, Davis, CA 95616, USAAdrienne Burke - USDA-ARS Wheat Genetics, Quality, Physiology and Disease Research Unit, Washington State University, Pullman, WA 99164-6420, USADeven Robert See - USDA-ARS Wheat Genetics, Quality, Physiology and Disease Research Unit, Washington State University, Pullman, WA 99164-6420, USADaniel Z Skinner - Crop and Soil Sciences Department, Washington State University, Pullman, WA 99164, USAJorge Dubcovsky - Department of Plant Sciences, University of California, Davis, CA 95616, USAKimberly Garland Campbell - Crop and Soil Sciences Department, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Theoretical and applied genetics, Vol.127(5), pp.1183-1197
- Academic Unit
- Crop and Soil Sciences, Department of; Pharmaceutical Sciences, Department of; Plant Pathology, Department of
- Identifiers
- 99900546830301842
- Language
- English
- Resource Type
- Journal article