Journal article
Correlation between monoclonal antibody reactivity and expression of CD4 and CD8α genes in the horse
Veterinary immunology and immunopathology, Vol.42(1), pp.61-69
1994
Handle:
https://hdl.handle.net/2376/115260
PMID: 7526532
Abstract
Equine peripheral blood lymphocytes (PBL) were enriched by positive selection using panning with a mixture of monoclonal antibodies against putative equine CD4 (Equine Leucocyte Antigen Workshop antibodies WS 1 and WS 72), or CD8 molecules (Workshop antibodies WS 12, WS 49, and WS 74). RNA was extracted from CD4 enriched cells (99% purity), from CD8 enriched cells (69% purity), from peripheral blood lymphocytes, and from neonatal equine thymus. RNA extracted from equine granulocytes and from equine kidney served as negative control. The RNA was electrophoresed in agarose and transferred to nylon membranes. Northern blots were hybridized with human and mouse cDNA probes for CD4 and CD8α. The human CD4 probe detected a 2.9 kb RNA transcript in equine PBL, CD4 enriched lymphocytes, and thymocytes. The human CD8α probe detected a 2.0 kb transcript in RNA from equine CD8α enriched lymphocytes and thymocytes, but not from PBL or CD4 enriched lymphocytes. Mouse cDNA probes for CD4 and CD8 did not react with equine RNA. Results of hybridizations using the human probes support the assignment of the CD4 and CD8 specificities to the antibodies listed above. The results also suggest that the equine CD4 and CD8α genes are more closely related to the human than to the murine counterparts.
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Details
- Title
- Correlation between monoclonal antibody reactivity and expression of CD4 and CD8α genes in the horse
- Creators
- G Grünig - Equine Genetics Center, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USAD.P Barbis - Equine Genetics Center, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USAC.H Zhang - Equine Genetics Center, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USAW.C Davis - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USAD.P Lunn - Department of Clinical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USAD.F Antczak - Equine Genetics Center, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
- Publication Details
- Veterinary immunology and immunopathology, Vol.42(1), pp.61-69
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548240701842
- Language
- English
- Resource Type
- Journal article