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Covalent modification of cell surfaces with TLR agonists improves & directs immune stimulation
Journal article   Open access

Covalent modification of cell surfaces with TLR agonists improves & directs immune stimulation

Janine K Tom, Rock J Mancini and Aaron P Esser-Kahn
Chemical communications (Cambridge, England), Vol.49(83), pp.9618-9620
10/25/2013
Handle:
https://hdl.handle.net/2376/111293
PMCID: PMC4399865
PMID: 24022092
url
https://doi.org/10.1039/c3cc45468aView
Published (Version of record) Open

Abstract

Toll-Like Receptors - immunology Carcinoma, Lewis Lung - immunology NF-kappa B - immunology Oligodeoxyribonucleotides - chemistry Teichoic Acids - chemistry Toll-Like Receptors - agonists Carcinoma, Lewis Lung - drug therapy Animals Teichoic Acids - pharmacology Lipopolysaccharides - pharmacology Cell Line, Tumor Lipopolysaccharides - chemistry Oligodeoxyribonucleotides - pharmacology
We present a primary example of a cell surface modified with a synergistic combination of agonists to tune immune stimulation. A model cell line, Lewis Lung Carcinoma, was covalently modified with CpG-oligonucleotides and lipoteichoic acid, both Toll-like receptor (TLR) agonists. The immune-stimulating constructs provided greater stimulation of NF-κB in a model cell line and bone marrow-derived dendritic cells than the components unconjugated in solution.

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