Cre activity in fetal albCre mouse hepatocytes: Utility for developmental studies

Carla M Weisend; Jean A Kundert; Elena S Suvorova; Justin R Prigge; Edward E Schmidt

doi:10.1002/dvg.20568

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Cre activity in fetal albCre mouse hepatocytes: Utility for developmental studies

Journal article

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Cre activity in fetal albCre mouse hepatocytes: Utility for developmental studies

Carla M Weisend, Jean A Kundert, Elena S Suvorova, Justin R Prigge and Edward E Schmidt

Genesis (New York, N.Y. : 2000), Vol.47(12), pp.789-792

12/2009

DOI: https://doi.org/10.1002/dvg.20568

Handle:

https://hdl.handle.net/2376/104554

PMCID: PMC2828742

PMID: 19830819

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Abstract

Animals, Newborn

Gene Transfer Techniques

Green Fluorescent Proteins - metabolism

Liver - metabolism

Transgenes - genetics

Serum Albumin - genetics

Male

Mice, Transgenic

Green Fluorescent Proteins - genetics

Hepatocytes - metabolism

Liver - embryology

Promoter Regions, Genetic - genetics

Hepatocytes - cytology

Animals

In Situ Hybridization

Gene Expression Regulation, Developmental

Female

Integrases - metabolism

Liver - cytology

Mice

Integrases - genetics

Microscopy, Fluorescence

The albCre transgene, having Cre recombinase driven by the serum albumin (alb) gene promoter, is commonly used to generate adult mice having reliable hepatocyte-specific recombination of loxP-flanked ("floxed") alleles. Based on previous studies, it has been unclear whether albCre transgenes are also reliable in fetal and juvenile mice. Perinatal liver undergoes a dynamic transition from being predominantly hematopoietic to predominantly hepatic. We evaluated Cre activity during this transition in albCre mice using a sensitive two-color fluorescent reporter system. From fetal through adult stages, in situ patterns of Cre-dependent recombination of the reporter closely matched expression of endogenous Alb mRNA or protein, indicating most or all hepatocytes, including those in fetal and juvenile livers, had expressed Cre and recombined the reporter. Our results indicate the albCre transgene is effective in converting simple floxed alleles in fetal and neonatal mice and is an appropriate tool for studies on hepatocyte development.

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Title: Cre activity in fetal albCre mouse hepatocytes: Utility for developmental studies
Creators: Carla M Weisend - Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59718, USA
Jean A Kundert
Elena S Suvorova
Justin R Prigge
Edward E Schmidt
Publication Details: Genesis (New York, N.Y. : 2000), Vol.47(12), pp.789-792
Academic Unit: UNKNOWN
Publisher: United States
Grant note: R01 AI055739-05 / NIAID NIH HHS R01 AI055739 / NIAID NIH HHS
Identifiers: 99900546652801842
Language: English
Resource Type: Journal article