Journal article
Crystal structure of a DNA decamer containing a cis-syn thymine dimer
Proceedings of the National Academy of Sciences - PNAS, Vol.99(25), pp.15965-15970
12/10/2002
Handle:
https://hdl.handle.net/2376/109034
PMCID: PMC138548
PMID: 12456887
Abstract
It is well known that exposure to UV induces DNA damage, which is the first step in mutagenesis and a major cause of skin cancer. Among a variety of photoproducts, cyclobutane-type pyrimidine photodimers (CPD) are the most abundant primary lesion. Despite its biological importance, the precise relationship between the structure and properties of DNA containing CPD has remained to be elucidated. Here, we report the free (unbound) crystal structure of duplex DNA containing a CPD lesion at a resolution of 2.0 Å. Our crystal structure shows that the overall helical axis bends ≈30° toward the major groove and unwinds ≈9°, in remarkable agreement with some previous theoretical and experimental studies. There are also significant differences in local structure compared with standard B-DNA, including pinching of the minor groove at the 3′ side of the CPD lesion, a severe change of the base pair parameter in the 5′ side, and serious widening of both minor and major groves both 3′ and 5′ of the CPD. Overall, the structure of the damaged DNA differs from undamaged DNA to an extent that DNA repair proteins may recognize this conformation, and the various components of the replicational and transcriptional machinery may be interfered with due to the perturbed local and global structure.
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Details
- Title
- Crystal structure of a DNA decamer containing a cis-syn thymine dimer
- Creators
- HaJeung Park - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660Kaijiang Zhang - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660Yingjie Ren - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660Sourena Nadji - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660Nanda Sinha - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660John-Stephen Taylor - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660ChulHee Kang - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.99(25), pp.15965-15970
- Academic Unit
- Chemistry, Department of
- Publisher
- National Academy of Sciences
- Identifiers
- 99900547292101842
- Language
- English
- Resource Type
- Journal article