Journal article
Ctk Complex-Mediated Regulation of Histone Methylation by COMPASS
Molecular and cellular biology, Vol.27(2), pp.709-720
01/15/2007
Handle:
https://hdl.handle.net/2376/110514
PMCID: PMC1800791
PMID: 17088385
Abstract
ABSTRACT A comparative global proteomic screen identified factors required for COMPASS (complex of proteins associated with Set1)-mediated mono-, di-, and trimethylation of the fourth lysine of histone H3 (H3K4), which included components of a cyclin-dependent protein kinase (Ctk complex) that phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II (Pol II). Our results indicate that histone H3K4 methylation levels are regulated by the Ctk1, Ctk2, and Ctk3 components of the Ctk complex. We show that loss of Ctk1 kinase activity results in reduced histone H3K4 monomethylation levels, followed by a global increase in histone H3K4 trimethylation levels on chromatin. Ctk1 loss does not appear to have a substantial effect on histone H2B monoubiquitination levels or COMPASS and Paf1 complex phosphorylation. Our chromatin immunoprecipitation studies demonstrate that histone H3 eviction during active transcription is decelerated in a CTK1 deletion strain in response to reduced levels of Pol II recruitment. Our in vitro studies show that the onset of monomethylation on an unmethylated histone H3 by COMPASS is virtually immediate, while the onset of trimethylation occurs upon extended time of association between the histone tail and COMPASS. Our study suggests a role for the Ctk complex in the regulation of the pattern of H3K4 mono-, di-, and trimethylation via COMPASS.
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Details
- Title
- Ctk Complex-Mediated Regulation of Histone Methylation by COMPASS
- Creators
- Adam Wood - Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, Missouri 63104Abhijit Shukla - Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, 1245 Lincoln Drive, Carbondale, Illinois 62901Jessica Schneider - Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, Missouri 63104Jung Shin Lee - Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, Missouri 63104Julie D Stanton - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Tiffany Dzuiba - Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, Missouri 63104Selene K Swanson - Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110Laurence Florens - Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110Michael P Washburn - Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110John Wyrick - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Sukesh R Bhaumik - Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, 1245 Lincoln Drive, Carbondale, Illinois 62901Ali Shilatifard - Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, Missouri 63104, Saint Louis University Cancer Center, Saint Louis University School of Medicine, St. Louis, Missouri 63104
- Publication Details
- Molecular and cellular biology, Vol.27(2), pp.709-720
- Academic Unit
- Molecular Biosciences, School of
- Identifiers
- 99900547376201842
- Language
- English
- Resource Type
- Journal article