Cullins 3a and 3b assemble with members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form essential ubiquitin-protein ligases (E3s) in Arabidopsis

Derek J Gingerich; Jennifer M Gagne; Donald W Salter; Hanjo Hellmann; Mark Estelle; Ligeng Ma; Richard D Vierstra

doi:10.1074/jbc.M413247200

Back

Cullins 3a and 3b assemble with members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form essential ubiquitin-protein ligases (E3s) in Arabidopsis

Journal article

Open access

Peer reviewed

Cullins 3a and 3b assemble with members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form essential ubiquitin-protein ligases (E3s) in Arabidopsis

Derek J Gingerich, Jennifer M Gagne, Donald W Salter, Hanjo Hellmann, Mark Estelle, Ligeng Ma and Richard D Vierstra

The Journal of biological chemistry, Vol.280(19), pp.18810-18821

05/13/2005

DOI: https://doi.org/10.1074/jbc.M413247200

Handle:

https://hdl.handle.net/2376/109011

PMID: 15749712

Files and links (1)

url

https://doi.org/10.1074/jbc.M413247200View

Published (Version of record) Open

Abstract

Multigene Family

Oligonucleotide Array Sequence Analysis

Humans

Molecular Sequence Data

Phylogeny

Arabidopsis Proteins

Codon

Gene Expression Regulation, Plant

Carrier Proteins - chemistry

Protein Structure, Tertiary

Amino Acid Sequence

Ubiquitin - chemistry

Reverse Transcriptase Polymerase Chain Reaction

Ubiquitin-Protein Ligases - chemistry

Amino Acid Motifs

Arabidopsis - metabolism

Sequence Homology, Amino Acid

Homozygote

DNA - chemistry

Phenotype

Animals

Cullin Proteins

Models, Biological

Protein Isoforms

Protein Binding

Heterozygote

Mutation

Selective modification of proteins by ubiquitination is directed by diverse families of ubiquitin-protein ligases (or E3s). A large collection of E3s use Cullins (CULs) as scaffolds to form multisubunit E3 complexes in which the CUL binds a target recognition subcomplex and the RBX1 docking protein, which delivers the activated ubiquitin moiety. Arabidopsis and rice contain a large collection of CUL isoforms, indicating that multiple CUL-based E3s exist in plants. Here we show that Arabidopsis CUL3a and CUL3b associate with RBX1 and members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form BTB E3s. Eighty genes encoding BTB domain-containing proteins were identified in the Arabidopsis genome, indicating that a diverse array of BTB E3s is possible. In addition to the BTB domain, the encoded proteins also contain various other interaction motifs that likely serve as target recognition elements. DNA microarray analyses show that BTB genes are expressed widely in the plant and that tissue-specific and isoform-specific patterns exist. Arabidopsis defective in both CUL3a and CUL3b are embryo-lethal, indicating that BTB E3s are essential for plant development.

Metrics

Details

Title: Cullins 3a and 3b assemble with members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form essential ubiquitin-protein ligases (E3s) in Arabidopsis
Creators: Derek J Gingerich - Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, USA
Jennifer M Gagne
Donald W Salter
Hanjo Hellmann
Mark Estelle
Ligeng Ma
Richard D Vierstra
Publication Details: The Journal of biological chemistry, Vol.280(19), pp.18810-18821
Academic Unit: Biological Sciences, School of
Publisher: United States
Grant note: F32-GM68361 / NIGMS NIH HHS
Identifiers: 99900547104401842
Language: English
Resource Type: Journal article