Culture of Campylobacter jejuni with sodium deoxycholate induces virulence gene expression

Preeti Malik-Kale; Craig T Parker; Michael E Konkel

doi:10.1128/JB.01736-07

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Culture of Campylobacter jejuni with sodium deoxycholate induces virulence gene expression

Journal article

Open access

Peer reviewed

Culture of Campylobacter jejuni with sodium deoxycholate induces virulence gene expression

Preeti Malik-Kale, Craig T Parker and Michael E Konkel

Journal of bacteriology, Vol.190(7), pp.2286-2297

04/2008

DOI: https://doi.org/10.1128/JB.01736-07

Handle:

https://hdl.handle.net/2376/106122

PMCID: PMC2293209

PMID: 18223090

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Abstract

Cell Line

Transcription, Genetic - drug effects

Porins - genetics

Campylobacter jejuni - pathogenicity

Oligonucleotide Array Sequence Analysis

Humans

Bacterial Proteins - genetics

Reverse Transcriptase Polymerase Chain Reaction

Antigens, Bacterial - genetics

Promoter Regions, Genetic - genetics

Campylobacter jejuni - drug effects

Gene Expression Regulation, Bacterial - drug effects

Deoxycholic Acid - pharmacology

Virulence - genetics

Bacterial Adhesion - drug effects

Campylobacter jejuni - genetics

Campylobacter jejuni, a spiral-shaped gram-negative bacterium, is a leading bacterial cause of human food-borne illness. Acute disease is associated with C. jejuni invasion of the intestinal epithelium. Further, maximal host cell invasion requires the secretion of proteins termed Campylobacter invasion antigens (Cia). As bile acids are known to alter the pathogenic behavior of other gastrointestinal pathogens, we hypothesized that the virulence potential of Campylobacter may be triggered by the bile acid deoxycholate (DOC). In support of this hypothesis, culturing C. jejuni with a physiologically relevant concentration of DOC significantly altered the kinetics of cell invasion, as shown by gentamicin protection assays. In contrast to C. jejuni harvested from Mueller-Hinton (MH) agar plates, C. jejuni harvested from MH agar plates supplemented with DOC secreted the Cia proteins, as judged by metabolic labeling experiments. DOC was also found to induce the expression of the ciaB gene, as determined by beta-galactosidase reporter, real-time reverse transcription-PCR, and microarray analyses. Microarray analysis further revealed that DOC induced the expression of virulence genes (ciaB, cmeABC, dccR, and tlyA). In summary, we demonstrated that it is possible to enhance the pathogenic behavior of C. jejuni by modifying the culture conditions. These results provide a foundation for identifying genes expressed by C. jejuni in response to in vivo-like culture conditions.

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Title: Culture of Campylobacter jejuni with sodium deoxycholate induces virulence gene expression
Creators: Preeti Malik-Kale - School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA
Craig T Parker
Michael E Konkel
Publication Details: Journal of bacteriology, Vol.190(7), pp.2286-2297
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Identifiers: 99900546748601842
Language: English
Resource Type: Journal article