Cultured renal epithelial cells from birds and mice: enhanced resistance of avian cells to oxidative stress and DNA damage

C E Ogburn; S N Austad; D J Holmes; J V Kiklevich; K Gollahon; P S Rabinovitch; G M Martin

doi:10.1093/gerona/53A.4.B287

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Cultured renal epithelial cells from birds and mice: enhanced resistance of avian cells to oxidative stress and DNA damage

Journal article

Open access

Peer reviewed

Cultured renal epithelial cells from birds and mice: enhanced resistance of avian cells to oxidative stress and DNA damage

C E Ogburn, S N Austad, D J Holmes, J V Kiklevich, K Gollahon, P S Rabinovitch and G M Martin

The journals of gerontology. Series A, Biological sciences and medical sciences, Vol.53(4), pp.B287-B292

07/1998

DOI: https://doi.org/10.1093/gerona/53A.4.B287

Handle:

https://hdl.handle.net/2376/113385

PMID: 18314559

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Abstract

Oxygen - pharmacology

Kidney - drug effects

Oxidative Stress

Mice, Inbred C57BL

Cells, Cultured

Hydrogen Peroxide - pharmacology

Birds

Kidney - cytology

Kidney - radiation effects

Animals

Flow Cytometry

Analysis of Variance

Mice

DNA Damage

Paraquat - pharmacology

Longevity - physiology

Radiation, Ionizing

Current mechanistic theories of aging would predict that many species of birds, given their unusually high metabolic rates, body temperatures, and blood sugar levels, should age more rapidly than mammals of comparable size. On the contrary, many avian species display unusually long life spans. This finding suggests that cells and tissues from some avian species may enjoy unusually robust and/or unique protective mechanisms against fundamental aging processes, including a relatively high resistance to oxidative stress. We therefore compared the sensitivities of presumptively homologous epithelial somatic cells derived from bird and mouse kidneys to various forms of oxidative stress. When compared to murine cells, we found enhanced resistance of avian cells from three species (budgerigars, starlings, canaries) to 95% oxygen, hydrogen peroxide, paraquat, and gamma-radiation. Differential resistance to 95% oxygen was demonstrated with both replicating and quiescent cultures. Hydrogen peroxide was shown to induce DNA single-strand breaks. There were fewer breaks in avian cells than in mouse cells when similarly challenged.

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Title: Cultured renal epithelial cells from birds and mice: enhanced resistance of avian cells to oxidative stress and DNA damage
Creators: C E Ogburn - Department of Pathology, University of Washington, Seattle 98195-7470, USA
S N Austad
D J Holmes
J V Kiklevich
K Gollahon
P S Rabinovitch
G M Martin
Publication Details: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol.53(4), pp.B287-B292
Academic Unit: Center for Reproductive Biology
Publisher: United States
Grant note: AG01751 / NIA NIH HHS
Identifiers: 99900547787101842
Language: English
Resource Type: Journal article