Journal article
Cytokine (IL16) and tyrphostin actions on ovarian primordial follicle development
Reproduction (Cambridge, England), Vol.148(3), pp.321-331
09/2014
Handle:
https://hdl.handle.net/2376/109460
PMCID: PMC4110175
PMID: 24970835
Abstract
An ovarian follicle is composed of an oocyte and surrounding theca and granulosa cells. Oocytes are stored in an arrested state within primordial follicles until they are signaled to re-initiate development by undergoing primordial-to-primary follicle transition. Previous gene bionetwork analyses of primordial follicle development identified a number of critical cytokine signaling pathways and genes potentially involved in the process. In the current study, candidate regulatory genes and pathways from the gene network analyses were tested for their effects on the formation of primordial follicles (follicle assembly) and on primordial follicle transition using whole ovary organ culture experiments. Observations indicate that the tyrphostin inhibitor (E)-2-benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one increased follicle assembly significantly, supporting a role for the MAPK signaling pathway in follicle assembly. The cytokine interleukin 16 (IL16) promotes primordial-to-primary follicle transition as compared with the controls, where as Delta-like ligand 4 (DLL4) and WNT-3A treatments have no effect. Immunohistochemical experiments demonstrated the localization of both the cytokine IL16 and its receptor CD4 in the granulosa cells surrounding each oocyte within the ovarian follicle. The tyrphostin LDN193189 (LDN) is an inhibitor of the bone morphogenic protein receptor 1 within the TGFB signaling pathway and was found to promote the primordial-to-primary follicle transition. Observations support the importance of cytokines (i.e., IL16) and cytokine signaling pathways in the regulation of early follicle development. Insights into regulatory factors affecting early primordial follicle development are provided that may associate with ovarian disease and translate to improved therapy in the future.
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Details
- Title
- Cytokine (IL16) and tyrphostin actions on ovarian primordial follicle development
- Creators
- Amanda Feeney - School of Biological SciencesCenter for Reproductive Biology, Washington State University, Pullman, Washington 99164-4236, USAEric Nilsson - School of Biological SciencesCenter for Reproductive Biology, Washington State University, Pullman, Washington 99164-4236, USAMichael K Skinner - School of Biological SciencesCenter for Reproductive Biology, Washington State University, Pullman, Washington 99164-4236, USA skinner@wsu.edu
- Publication Details
- Reproduction (Cambridge, England), Vol.148(3), pp.321-331
- Academic Unit
- Biological Sciences, School of
- Publisher
- Bioscientifica Ltd; England
- Number of pages
- 11
- Grant note
- R01 ES012974 / NIEHS NIH HHS R01 HD048898 / NICHD NIH HHS
- Identifiers
- 99900547290201842
- Language
- English
- Resource Type
- Journal article