Journal article
Cytokine RNA expression in an equine CD4 + subset differentiated by expression of a novel 46-kDa surface protein
Veterinary immunology and immunopathology, Vol.56(3), pp.191-204
1997
Handle:
https://hdl.handle.net/2376/106318
PMID: 9223225
Abstract
Two monoclonal antibodies (MAb), HB65A (IgG2a) and HB86A (IgGI), recognize a unique cell surface molecule on equine T-lymphocytes. The molecule, designated EqWC4, identified by these MAbs is present on a subpopulation of CD4+ equine lymphocytes (6.3–10.2% of Arabian lymphocytes CD4+ WC4+) and a smaller population of CD8+ lymphocytes (0.5% to 1.2% of Arabian lymphocytes CD8+ WC4+). EqWC4 is absent from B-lymphocytes, granulocytes, and macrophages. Both MAbs bound to a 46-kDa protein following immunoprecipitation reactions with lysates of surface labeled thymocytes. Immunoaffinity purification using HB65A yielded two molecules of 46 kDa and 52 kDa under reducing conditions and a third 92-kDa molecule was present in nonreduced conditions. Activation by mitogen did not increase expression of EqWC4 on equine lymphocytes. Lymphocytes from Arabian, Pony, and Thoroughbred breeds showed a common distribution of EqWC4 among leukocytes. However, there were significantly fewer Pony lymphocytes bound to HB65A and HB86A when compared to Arabian and Thoroughbred breeds. Using reverse transcriptase-polymerase chain reaction (RT-PCR), magnetically enriched populations (to 80% of cells isolated) of EqWC4+ lymphocytes expressed a cytokine RNA profile dominated by -interleukin2 (IL-2) and interferon-gamma (IFN-γ) for unstimulated cells. Upon mitogen stimulation, IL-4 was also expressed at low levels while the IL-2 levels decreased and the IFN-γ levels increased relative to unstimulated cells. EqWC4 is similar to CD28 in molecular weight and its formation of dimers and could therefore be the equine orthologue. However, because of the differences in CD28 expression, EqWC4 probably represents a previously uncharacterized equine lymphocyte marker.
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Details
- Title
- Cytokine RNA expression in an equine CD4 + subset differentiated by expression of a novel 46-kDa surface protein
- Creators
- K M Byrne - Department of Animal Science, College of Agriculture and Home Economics, Washington State University, Pullman, WA 99164-6310, USAW C Davis - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAM.A Holmes - Department of Clinical Veterinary Medicine, Madingley Road, Cambridge CB3 OES, UKA.L Brassfield - Department of Animal Science, College of Agriculture and Home Economics, Washington State University, Pullman, WA 99164-6310, USAT.C McGuire - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA
- Publication Details
- Veterinary immunology and immunopathology, Vol.56(3), pp.191-204
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546826901842
- Language
- English
- Resource Type
- Journal article