Journal article
Cytokine mRNA induction by interleukin-1β or tumor necrosis factor α in vitro and in vivo
Brain research, Vol.1226, pp.89-98
2008
Handle:
https://hdl.handle.net/2376/116263
PMCID: PMC2642478
PMID: 18620339
Abstract
Hypothalamic and cortical mRNA levels for cytokines such as interleukin-1β (IL1β), tumor necrosis factor alpha (TNFα), nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) are impacted by systemic treatments of IL1β and TNFα. To investigate the time course of the effects of IL1β and TNFα on hypothalamic and cortical cytokine gene expression, we measured mRNA levels for IL1β, TNFα, interleukin-6 (IL-6), interleukin-10 (IL-10), IL1 receptor 1, BDNF, NGF, and glutamate decarboxylase-67
in vitro using hypothalamic and cortical primary cultures. IL1β and TNFα mRNA levels increased significantly in a dose-dependent fashion after exposure to either IL1β or TNFα. IL1β increased IL1β mRNA in both the hypothalamic and cortical cultures after 2–6 h while TNFα mRNA increased significantly within 30 min and continued to rise up to 2–6 h. Most of the other mRNAs showed significant changes independent of dose
in vitro.
In vivo, intracerebroventricular (icv) injection of IL1β or TNFα also significantly increased IL1β, TNFα and IL6 mRNA levels in the hypothalamus and cortex. IL1β icv, but not TNFα, increased NGF mRNA levels in both these areas. Results support the hypothesis that centrally active doses of IL1β and TNFα enhance their own mRNA levels as well as affect mRNA levels for other neuronal growth factors.
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Details
- Title
- Cytokine mRNA induction by interleukin-1β or tumor necrosis factor α in vitro and in vivo
- Creators
- Ping Taishi - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Programs in Neuroscience, Washington State University, Pullman, WA, USALynn Churchill - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Programs in Neuroscience, Washington State University, Pullman, WA, USAAlok De - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Programs in Neuroscience, Washington State University, Pullman, WA, USAFerenc Obal - Endocrine Unit, A. Szent-Gyorgyi Center, University of Szeged, Szeged, HungaryJames M Krueger - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Programs in Neuroscience, Washington State University, Pullman, WA, USA
- Publication Details
- Brain research, Vol.1226, pp.89-98
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548399401842
- Language
- English
- Resource Type
- Journal article