DMRT1 promotes oogenesis by transcriptional activation of Stra8 in the mammalian fetal ovary

Anthony D Krentz; Mark W Murphy; Aaron L Sarver; Michael D Griswold; Vivian J Bardwell; David Zarkower

doi:10.1016/j.ydbio.2011.05.658

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DMRT1 promotes oogenesis by transcriptional activation of Stra8 in the mammalian fetal ovary

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DMRT1 promotes oogenesis by transcriptional activation of Stra8 in the mammalian fetal ovary

Anthony D Krentz, Mark W Murphy, Aaron L Sarver, Michael D Griswold, Vivian J Bardwell and David Zarkower

Developmental biology, Vol.356(1), pp.63-70

08/01/2011

DOI: https://doi.org/10.1016/j.ydbio.2011.05.658

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https://hdl.handle.net/2376/114544

PMCID: PMC3131262

PMID: 21621532

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Abstract

Stra8

DM domain

Ovary

Meiosis

Dmrt1

Dmrt1 belongs to the DM domain gene family of conserved sexual regulators. In the mouse Dmrt1 is expressed in the genital ridge (the gonadal primordium) in both sexes and then becomes testis-specific shortly after sex determination. The essential role of DMRT1 in testicular differentiation is well established, and includes transcriptional repression of the meiotic inducer Stra8. However Dmrt1 mutant females are fertile and the role of Dmrt1 in the ovary has not been studied. Here we show in the mouse that most Dmrt1 mutant germ cells in the fetal ovary have greatly reduced expression of STRA8, and fail to properly localize SYCP3 and γH2AX during meiotic prophase. Lack of DMRT1 in the fetal ovary results in the formation of many fewer primordial follicles in the juvenile ovary, although these are sufficient for fertility. Genome-wide chromatin immunoprecipitiation (ChIP-chip) and quantitative ChIP (qChIP) combined with mRNA expression profiling suggests that transcriptional activation of Stra8 in fetal germ cells is the main function of DMRT1 in females, and that this regulation likely is direct. Thus DMRT1 controls Stra8 sex-specifically, activating it in the fetal ovary and repressing it in the adult testis. ► DMRT1 binds to and activates transcription of Stra8 in the fetal ovary. ► Dmrt1-/- germ cells fail to properly localize SYCP3 and γH2AX during prophase I. ► Dmrt1-/- females have a reduction in the number of ovarian follicles postnatally, but are fertile

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Title: DMRT1 promotes oogenesis by transcriptional activation of Stra8 in the mammalian fetal ovary
Creators: Anthony D Krentz - Dept. of Genetics, Cell Biology, and Development, and Developmental Biology Center, University of Minnesota, Minneapolis, MN, USA
Mark W Murphy - Dept. of Genetics, Cell Biology, and Development, and Developmental Biology Center, University of Minnesota, Minneapolis, MN, USA
Aaron L Sarver - Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
Michael D Griswold - School of Molecular Biosciences, Washington State University, Pullman, WA, USA
Vivian J Bardwell - Dept. of Genetics, Cell Biology, and Development, and Developmental Biology Center, University of Minnesota, Minneapolis, MN, USA
David Zarkower - Dept. of Genetics, Cell Biology, and Development, and Developmental Biology Center, University of Minnesota, Minneapolis, MN, USA
Publication Details: Developmental biology, Vol.356(1), pp.63-70
Academic Unit: Molecular Biosciences, School of
Publisher: Elsevier Inc
Identifiers: 99900547997801842
Language: English
Resource Type: Journal article