Journal article
DNA Sequence and Comparison of Virulence Plasmids from Rhodococcus equi ATCC 33701 and 103
Infection and immunity, Vol.68(12), pp.6840-6847
12/2000
Handle:
https://hdl.handle.net/2376/110241
PMCID: PMC97788
PMID: 11083803
Abstract
The virulence plasmids of the equine virulent strains
Rhodococcus equi
ATCC 33701 and 103 were sequenced, and their genetic structure was analyzed. p33701 was 80,610 bp in length, and p103 was 1 bp shorter; their sequences were virtually identical. The plasmids contained 64 open reading frames (ORFs), 22 of which were homologous with genes of known function and 3 of which were homologous with putative genes of unknown function in other species. Putative functions were assigned to five ORFs based on protein family characteristics. The most striking feature of the virulence plasmids was the presence of a 27,536-bp pathogenicity island containing seven virulence-associated protein (
vap
) genes, including
vapA
. These
vap
genes have extensive homology to
vapA
, which encodes a thermoregulated and surface-expressed protein. The pathogenicity island contained a LysR family transcriptional regulator and a two-component response regulator upstream of six of the
vap
genes. The
vap
genes were present as a cluster of three (
vapA
,
vapC
, and
vapD
), as a pair (
vapE
and
vapF
), or individually (
vapG
;
vapH
). A region of extensive direct repeats of unknown function, possibly associated with thermoregulation, was present immediately upstream of the clustered and the paired genes but not the individual
vap
genes. There was extensive homology among the C-terminal halves of all
vap
genes but not generally among the N-terminal halves. The remainder of the plasmid consisted of a large region which appears to be associated with conjugation functions and a large region which appears to be associated with replication and partitioning functions.
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Details
- Title
- DNA Sequence and Comparison of Virulence Plasmids from Rhodococcus equi ATCC 33701 and 103
- Creators
- Shinji Takai - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Stephen A Hines - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Tsutomu Sekizaki - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Vivian M Nicholson - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Debra A Alperin - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Makoto Osaki - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Daisuke Takamatsu - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Mutsu Nakamura - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Kayo Suzuki - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Nobuko Ogino - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Tsutomu Kakuda - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628Hanhong Dan - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628John F Prescott - Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628
- Publication Details
- Infection and immunity, Vol.68(12), pp.6840-6847
- Academic Unit
- Veterinary Medicine, College of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547220501842
- Language
- English
- Resource Type
- Journal article