Journal article
DNA Vaccine Construct Incorporating Intercellular Trafficking and Intracellular Targeting Motifs Effectively Primes and Induces Memory B- and T-Cell Responses in Outbred Animals
Clinical and vaccine immunology, Vol.14(3), pp.304-311
03/2007
Handle:
https://hdl.handle.net/2376/110640
PMCID: PMC1828862
PMID: 17215335
Abstract
We developed a vaccine construct in which a BVP22 domain and an invariant-chain major histocompatibility complex class II-targeting motif capable of enhancing dendritic cell antigen uptake and presentation were fused to a sequence encoding a B- and T-cell antigen from the
Anaplasma marginale
major surface protein 1a and tested whether this construct would prime and expand immune responses in outbred calves. A single inoculation with this construct effectively primed the immune responses, as demonstrated by a significant enhancement of CD4
+
T-cell proliferation compared to that in calves identically inoculated but inoculated with a DNA construct lacking the targeting domains and compared to that in calves inoculated with an empty vector. These proliferative responses were mirrored by priming and expansion of gamma interferon-positive CD4
+
T cells and immunoglobulin G responses against the linked B-cell epitope. Priming by the single immunization induced memory that underwent rapid recall following reexposure to the antigen. These results demonstrate that DNA vaccines targeting key intercellular and intracellular events significantly enhance priming and expansion and support the feasibility of single-dose DNA immunization in outbred populations.
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Details
- Title
- DNA Vaccine Construct Incorporating Intercellular Trafficking and Intracellular Targeting Motifs Effectively Primes and Induces Memory B- and T-Cell Responses in Outbred Animals
- Creators
- Waithaka Mwangi - Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Wendy C Brown - Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Gary A Splitter - Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Christopher J Davies - Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Chris J Howard - Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Jayne C Hope - Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Yoko Aida - Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Yan Zhuang - Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Beverly J Hunter - Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Guy H Palmer - Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164
- Publication Details
- Clinical and vaccine immunology, Vol.14(3), pp.304-311
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547648701842
- Language
- English
- Resource Type
- Journal article