DNA repair of a single UV photoproduct in a designed nucleosome

Joseph V Kosmoski; Eric J Ackerman; Michael J Smerdon

doi:10.1073/pnas.181073398

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DNA repair of a single UV photoproduct in a designed nucleosome

Journal article

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DNA repair of a single UV photoproduct in a designed nucleosome

Joseph V Kosmoski, Eric J Ackerman and Michael J Smerdon

Proceedings of the National Academy of Sciences - PNAS, Vol.98(18), pp.10113-10118

08/28/2001

DOI: https://doi.org/10.1073/pnas.181073398

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https://hdl.handle.net/2376/107553

PMCID: PMC56924

PMID: 11517308

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Abstract

Biological Sciences

Eukaryotic DNA repair enzymes must interact with the architectural hierarchy of chromatin. The challenge of finding damaged DNA complexed with histone proteins in nucleosomes is complicated by the need to maintain local chromatin structures involved in regulating other DNA processing events. The heterogeneity of lesions induced by DNA-damaging agents has led us to design homogeneously damaged substrates to directly compare repair of naked DNA with that of nucleosomes. Here we report that nucleotide excision repair in Xenopus nuclear extracts can effectively repair a single UV radiation photoproduct located 5 bases from the dyad center of a positioned nucleosome, although the nucleosome is repaired at about half the rate at which the naked DNA fragment is. Extract repair within the nucleosome is >50-fold more rapid than either enzymatic photoreversal or endonuclease cleavage of the lesion in vitro . Furthermore, nucleosome formation occurs (after repair) only on damaged naked DNA (165-bp fragments) during a 1-h incubation in these extracts, even in the presence of a large excess of undamaged DNA. This is an example of selective nucleosome assembly by Xenopus nuclear extracts on a short linear DNA fragment containing a DNA lesion.

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Title: DNA repair of a single UV photoproduct in a designed nucleosome
Creators: Joseph V Kosmoski - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660; and
Eric J Ackerman - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660; and
Michael J Smerdon - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660; and
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.98(18), pp.10113-10118
Academic Unit: Molecular Biosciences, School of
Publisher: The National Academy of Sciences
Identifiers: 99900547165501842
Language: English
Resource Type: Journal article