Journal article
De Novo Sequencing and Transcriptome Analysis of Pleurotus eryngii subsp. tuoliensis (Bailinggu) Mycelia in Response to Cold Stimulation
Molecules (Basel, Switzerland), Vol.21(5), p.560
05/17/2016
Handle:
https://hdl.handle.net/2376/115536
PMCID: PMC6273410
PMID: 27196889
Abstract
Cold stimulation of Bailinggu's mycelia is the main factor that triggers primordia initiation for successful production of fruiting bodies under commercial cultivation. Yet, the molecular-level mechanisms involved in mycelia response to cold stimulation are still unclear. Here, we performed comparative transcriptomic analysis using RNA-Seq technology to better understand the gene expression regulation during different temporal stages of cold stimulation in Bailinggu. A total of 21,558 Bailinggu mycelia unigenes were de novo assembled and annotated from four libraries (control at 25 °C, plus cold stimulation treatments at -3 °C for a duration of 1-2 days, 5-6 days, and 9-10 days). GO and KEGG pathway analysis indicated that functional groups of differentially expressed unigenes associated with cell wall and membrane stabilization, calcium signaling and mitogen-activated protein kinases (MAPK) pathways, and soluble sugars and protein biosynthesis and metabolism pathways play a vital role in Bailinggu's response to cold stimulation. Six hundred and seven potential EST-based SSRs loci were identified in these unigenes, and 100 EST-SSR primers were randomly selected for validation. The overall polymorphism rate was 92% by using 10 wild strains of Bailinggu. Therefore, these results can serve as a valuable resource for a better understanding of the molecular mechanisms associated with Bailinggu's response to cold stimulation.
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- Title
- De Novo Sequencing and Transcriptome Analysis of Pleurotus eryngii subsp. tuoliensis (Bailinggu) Mycelia in Response to Cold Stimulation
- Creators
- Yong-Ping Fu - Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99163, USA. yongpingfu81@126.comYuan Liang - Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. liangyuan@big.ac.cnYue-Ting Dai - Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. daiyueting18@163.comChen-Tao Yang - China National GeneBank, Environmental Genomics, BGI, Shenzhen 518083, China. yangchentao@genomics.cnMing-Zheng Duan - Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. duanmingzheng@hotmail.comZhuo Zhang - Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. zzo1018@126.comSong-Nian Hu - Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. husn@big.ac.cnZhi-Wu Zhang - Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99163, USA. zhiwu.zhang@wsu.eduYu Li - Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. yuli966@126.com
- Publication Details
- Molecules (Basel, Switzerland), Vol.21(5), p.560
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- Switzerland
- Identifiers
- 99900547801801842
- Language
- English
- Resource Type
- Journal article