Death of serum-free mouse embryo cells caused by epidermal growth factor deprivation

C L Rawson; D T Loo; J R Duimstra; O R Hedstrom; E E Schmidt; D W Barnes

doi:10.1083/jcb.113.3.671

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Death of serum-free mouse embryo cells caused by epidermal growth factor deprivation

Journal article

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Peer reviewed

Death of serum-free mouse embryo cells caused by epidermal growth factor deprivation

C L Rawson, D T Loo, J R Duimstra, O R Hedstrom, E E Schmidt and D W Barnes

The Journal of cell biology, Vol.113(3), pp.671-680

05/1991

DOI: https://doi.org/10.1083/jcb.113.3.671

Handle:

https://hdl.handle.net/2376/115391

PMCID: PMC2288960

PMID: 2016341

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Abstract

Astrocytes - cytology

L-Lactate Dehydrogenase - metabolism

Cell Survival

Embryo, Mammalian

Cells, Cultured

RNA, Messenger - analysis

Cytoplasm - ultrastructure

Cell Nucleus - ultrastructure

Microscopy, Electron

Blood

Astrocytes - ultrastructure

G1 Phase

Animals

Culture Media

DNA - analysis

Astrocytes - chemistry

Mice

Polyribosomes - ultrastructure

Epidermal Growth Factor - pharmacology

Serum-free mouse embryo (SFME) cells, derived in medium in which serum is replaced with growth factors and other supplements, are proastroblasts that are acutely dependent on epidermal growth factor (EGF) for survival. Ultrastructurally, an early change found in SFME cells deprived of EGF was a loss of polysomes which sedimentation analysis confirmed to be a shift from polysomes to monosomes. The ribosomal shift was not accompanied by decreased steady-state level of cytoplasmic actin mRNA examined as an indicator of cellular mRNA level. With time the cells became small and severely degenerate and exhibited nuclear morphology characteristic of apoptosis. Genomic DNA isolated from cultures undergoing EGF deprivation-dependent cell death exhibited a pattern of fragmentation resulting from endonuclease activation characteristic of cells undergoing apoptosis or programmed cell death. Flow cytometric analysis indicated that cultures in the absence of EGF contained almost exclusively G1-phase cells. Some of the phenomena associated with EGF deprivation of SFME cells are similar to those observed upon NGF deprivation of nerve cells in culture, suggesting that these neuroectodermal-derived cell types share common mechanisms of proliferative control involving peptide growth factor-dependent survival.

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Title: Death of serum-free mouse embryo cells caused by epidermal growth factor deprivation
Creators: C L Rawson - Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503
D T Loo
J R Duimstra
O R Hedstrom
E E Schmidt
D W Barnes
Publication Details: The Journal of cell biology, Vol.113(3), pp.671-680
Academic Unit: UNKNOWN
Publisher: United States
Grant note: ES00210 / NIEHS NIH HHS 01226 / PHS HHS 07560 / PHS HHS
Identifiers: 99900547878701842
Language: English
Resource Type: Journal article