Journal article
Deficiency of Growth Hormone-Releasing Hormone Signaling Is Associated with Sleep Alterations in the Dwarf Rat
The Journal of neuroscience, Vol.21(8), pp.2912-2918
04/15/2001
Handle:
https://hdl.handle.net/2376/105501
PMCID: PMC6762541
PMID: 11306643
Abstract
The somatotropic axis, and particularly growth hormone-releasing hormone (GHRH), is implicated in the regulation of sleep–wake activity. To evaluate sleep in chronic somatotropic deficiency, sleep–wake activity was studied in dwarf (
dw/dw
) rats that are known to have a defective GHRH signaling mechanism in the pituitary and in normal Lewis rats, the parental strain of the
dw/dw
rats. In addition, expression of GHRH receptor (GHRH-R) mRNA in the hypothalamus/preoptic region and in the pituitary was also determined by means of reverse transcription-PCR, and GHRH content of the hypothalamus was measured. Hypothalamic/preoptic and pituitary GHRH-R mRNA levels were decreased in the
dw/dw
rats, indicating deficits in the central GHRHergic transmission. Hypothalamic GHRH content in
dw/dw
rats was also less than that found in Lewis rats. The
dw/dw
rats had less spontaneous nonrapid eye movement sleep (NREMS) (light and dark period) and rapid eye movement sleep (REMS) (light period) than did the control Lewis rats. After 4 hr of sleep deprivation, rebound increases in NREMS and REMS were normal in the
dw/dw
rat. As determined by fast Fourier analysis of the electroencephalogram (EEG), the sleep deprivation-induced enhancements in EEG slow-wave activity in the
dw/dw
rats were only one-half of the response in the Lewis rats. The results are compared with sleep findings previously obtained in GHRH-deficient transgenic mice. The alterations in NREMS are attributed to the defect in GHRH signaling, whereas the decreases in REMS might result from the growth hormone deficiency in the
dw/dw
rat.
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Details
- Title
- Deficiency of Growth Hormone-Releasing Hormone Signaling Is Associated with Sleep Alterations in the Dwarf Rat
- Creators
- Ferenc ObálJidong FangPing TaishiBalint KacsóhJanos GardiJames M Krueger
- Publication Details
- The Journal of neuroscience, Vol.21(8), pp.2912-2918
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Society for Neuroscience
- Identifiers
- 99900546661501842
- Language
- English
- Resource Type
- Journal article