Journal article
Degradation of oxalic acid by the mycoparasite Coniothyrium minitans plays an important role in interacting with Sclerotinia sclerotiorum
Environmental microbiology, Vol.16(8), pp.2591-2610
08/2014
Handle:
https://hdl.handle.net/2376/106972
PMID: 24467446
Abstract
Summary
Coniothyrium minitans (Cm) is a mycoparasite of the phytopathogenic fungus Sclerotinia sclerotiorum (Ss). Ss produces a virulence factor oxalic acid (OA) which is toxic to plants and also to Cm, and Cm detoxifies OA by degradation. In this study, two oxalate decarboxylase genes, Cmoxdc1 and Cmoxdc2, were cloned from Cm strain Chy‐1. OA and low pH induced expression of Cmoxdc1, but not Cmoxdc2. Cmoxdc1 was partially responsible for OA degradation, whereas Cmoxdc2 had no effect on OA degradation. Disruption of Cmoxdc1 in Cm reduced its ability to infect Ss in dual cultures where OA accumulated. Compared with Chy‐1, the Cmoxdc1‐disrupted mutants had reduced expression levels of two mycoparasitism‐related genes chitinase (Cmch1) and β‐1,3‐glucanase (Cmg1), and had no detectable activity of extracellular proteases in the presence of OA. On the other hand, the cultural filtrates of the Cmoxdc1‐disrupted mutants in OA‐amended media showed enhanced antifungal activity, possibly because of increased production of antifungal substances under acidic pH condition resulted from reduced Cmoxdc1‐mediated OA degradation. This study provides direct genetic evidence of OA degradation regulating mycoparasitism and antibiosis of Cm against Ss, and sheds light on the sophisticated strategies of Cm in interacting with metabolically active mycelia and dormant sclerotia of Ss.
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Details
- Title
- Degradation of oxalic acid by the mycoparasite Coniothyrium minitans plays an important role in interacting with Sclerotinia sclerotiorum
- Creators
- Li‐Mei Zeng - Huazhong Agricultural UniversityJing Zhang - Huazhong Agricultural UniversityYong‐Chao Han - Huazhong Agricultural UniversityLong Yang - Huazhong Agricultural UniversityMing‐de Wu - Huazhong Agricultural UniversityDao‐Hong Jiang - Huazhong Agricultural UniversityWeidong Chen - Washington State UniversityGuo‐Qing Li - Huazhong Agricultural University
- Publication Details
- Environmental microbiology, Vol.16(8), pp.2591-2610
- Academic Unit
- Plant Pathology, Department of
- Number of pages
- 20
- Grant note
- National Natural Science Foundation of China (31000877; 31272084) Agroscientific Research in the Public Interest (201103016) National ‘973’ Basic Research Program of China (2012cb114000)
- Identifiers
- 99900546963301842
- Language
- English
- Resource Type
- Journal article