Journal article
Depression of home cage wheel running is an objective measure of spontaneous morphine withdrawal in rats with and without persistent pain
Pharmacology, biochemistry and behavior, Vol.156, pp.10-15
05/2017
Handle:
https://hdl.handle.net/2376/104271
PMCID: PMC5484634
PMID: 28366799
Abstract
Opioid withdrawal in humans is often subtle and almost always spontaneous. In contrast, most preclinical studies precipitate withdrawal by administration of an opioid receptor antagonist such as naloxone. These animal studies rely on measurement of physiological symptoms (e.g., wet dog shakes) in the period immediately following naloxone administration. To more closely model the human condition, we tested the hypothesis that depression of home cage wheel running will provide an objective method to measure the magnitude and duration of spontaneous morphine withdrawal. Rats were allowed access to a running wheel in their home cage for 8days prior to implantation of two 75mg morphine or placebo pellets. The pellets were removed 3 or 5days later to induce spontaneous withdrawal. In normal pain-free rats, removal of the morphine pellets depressed wheel running for 48h compared to rats that had placebo pellets removed. Morphine withdrawal-induced depression of wheel running was greatly enhanced in rats with persistent inflammatory pain induced by injection of Complete Freund's Adjuvant (CFA) into the hindpaw. Removal of the morphine pellets following 3days of treatment depressed wheel running in these rats for over 6days. These data demonstrate that home cage wheel running provides an objective and more clinically relevant method to assess spontaneous morphine withdrawal compared to precipitated withdrawal in laboratory rats. Moreover, the enhanced withdrawal in rats with persistent inflammatory pain suggests that pain patients may be especially susceptible to opioid withdrawal.
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Details
- Title
- Depression of home cage wheel running is an objective measure of spontaneous morphine withdrawal in rats with and without persistent pain
- Creators
- Ram Kandasamy - Graduate Program in Neuroscience, Washington State University, Pullman, WA, USA. Electronic address: ram_kandasamy@wsu.eduAndrea T Lee - Department of Psychology, Washington State University Vancouver, Vancouver, WA, USAMichael M Morgan - Graduate Program in Neuroscience, Washington State University, Pullman, WA, USA; Department of Psychology, Washington State University Vancouver, Vancouver, WA, USA
- Publication Details
- Pharmacology, biochemistry and behavior, Vol.156, pp.10-15
- Academic Unit
- Psychology, Department of
- Publisher
- United States
- Grant note
- R03 NS095057 / NINDS NIH HHS
- Identifiers
- 99900546775501842
- Language
- English
- Resource Type
- Journal article