Journal article
Design and Construction of a Preparative-Scale Dynamic Field Gradient Focusing Apparatus
Biotechnology progress, Vol.24(2), pp.444-451
2008
Handle:
https://hdl.handle.net/2376/109525
PMCID: PMC2925538
PMID: 18225913
Abstract
A linear model is used to show that dynamic field gradient focusing (DFGF) can be scaled to preparative capacity, ~O (10 mgs). This paper explains how the preparative-scale DFGF apparatus was designed and fabricated. Scaled-down experiments and mathematical modeling guided material selection and design changes during construction to increase the probability that the prototype preparative-scale DFGF apparatus would perform as intended. The finished prototype successfully focused bovine hemoglobin from an initial concentration of 6.82 to 15 mg/mL and allowed for 86% recovery of injected protein.
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Details
- Title
- Design and Construction of a Preparative-Scale Dynamic Field Gradient Focusing Apparatus
- Creators
- Noah I Tracy - School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington 99164Zheng Huang - Protasis Corporation, Marlboro, Massachusetts, 01752Cornelius F Ivory - School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington 99164
- Publication Details
- Biotechnology progress, Vol.24(2), pp.444-451
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Identifiers
- 99900547282001842
- Language
- English
- Resource Type
- Journal article